珠江上游地区蚊虫乙型脑炎病毒核酸检测及PrM和E基因序列分析

doi:10.16431/j.cnki.1671-7236.2017.02.005

Abstract

Abstract:

This experiment was conducted to understand mosquito species, distribution of the main vector of Japanese encephalitis virus (JEV), JEV genotype and molecular characteristics in upper Pearl River region. We collected mosquito and Culicoides specimen in Shizong county, upper Pearl River region in July 2013, and these specimens were classified and identified. Meanwhile, JEV nucleotide were detected using PrM and E genes specific primers in 162 pools of mosquito and Culicoides specimen, and the positive specimens were sequencd and analyzed by bioinformatics software. Total 3 705 mosquitoes were collected from 4 collection spots, and the mosquitoes belong to 7 species in 4 generas: Culex, Anopheles, Aedes and Armigeres. In cattle pens, Anopheles Sinensis and Armigeres subalbatus were the dominant mosquito species. In sheep pens, Anopheles Sinensis and Armigeres subalbatus were the dominant mosquito species. And in pig pens, Culex tritaeniorhynchus, Anopheles Sinensis and Armigeres subalbatus were the dominant mosquito species. 4 of 162 pools mosquito and Culicoides were positive detecting by using PrM and E genes specific primers. Sequence analysis of JEV PrM and E genes results showed that 4 strains virus from Culex tritaeniorhynchus samples belonged to genotype Ⅰ JEV, and existing 15 amino acid difference sites between vaccine strain SA14-14-2 in the E gene protein, and 8 important sites which affected virus virulence did not mutant. These results suggested that there were many mosquito species in upper Pearl River region, and Culex tritaeniorhynchus, Anopheles Sinensis were dominant mosquito species in this region, and Culex tritaeniorhynchus was the main media of JEV in local region, genotypeⅠ JEV was popular in local media, and virulence of the pandemic strain did not reduce.

Key words: Japanese encephalitis virus; classification of mosquito; Culicoides; PrM gene; E gene

CLC Number:

R384.1

LV Shun-yan, WANG Jing-lin, LI Nan, HU Qi, HE Yu-wen, ZHAO De-hong, CHEN Hong-yu, LI Hua-chun. Nucleotide Detection of Mosquito Japanese Encephalitis Virus and Sequence Analysis of PrM and E Genes in Upper Pearl River Region[J]. , 2017, 44(2): 336-343.

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Nucleotide Detection of Mosquito Japanese Encephalitis Virus and Sequence Analysis of PrM and E Genes in Upper Pearl River Region

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