中国地鼠线粒体D-loop基因的克隆及序列分析

Abstract

Abstract: The primer was designed according to the closest animal published partial sequences, the PCR products were sequenced and determined. Combined with the known D-loop region sequence of other rodents,nucleotide composition were analyzed with DNAStar and genetic distance was analyzed with MEGA 4.1. The phylogenetic tree was constructed by neighbor-joining methods (NJ) and minimum-evolution methods (ME). The D-loop sequence of Chinese hamster had 867 bp, the nucleotide composition were relatively T(31.49%), C(26.07%), A(29.53%), and G(12.92%). The different clustering methods all showed a similar result. Chinese hamsters had the closest relationship with golden hamsters, but had relatively large difference from mice and rats, basically consistent with the traditional taxonomic status. D-loop gene might be genetic marker among species. The results of this study might be of importance for studies on evolution,structure and function of the mitochondria in Chinese hamsters.

Key words: Chinese hamster; mitochondrial DNA; D-loop gene; phylogenetic relationship

CLC Number:

S813.3

SONG Guo-hua, CHEN Chao-yang, PANG Wen-biao, GAO Ji-ping, YUE Wen-bin. Cloning and Sequence Analysis of Mitochondrial D-loop Gene in Chinese Hamster[J]. , 2013, 40(2): 126-129.

References

1 孙玉华,王伟,刘思阳,等. 中国胭脂鱼mtDNA 控制区遗传多样性分析[J]. 遗传学报,2002,29(9):787～790.
2 李长龙,卢领群,郭红刚,等.长爪沙鼠线粒体DNA控制区全序列测定及分析[J].中国比较医学杂志,2010,20(4):40～45.
3 李庆伟,马飞.鸟类分子进化与分子系统学[M].北京:科学出版社,2007.
4 宋国华,岳文斌,刘田福.中国地鼠线粒体Cyt b基因测序及其分子进化[J].中国实验动物学报,2008,16(2):142～147.
5 於建国,陈静波,多来提尼牙孜·依明,等.和田黑鸡mtDNA控制区D-loop序列分析[J]. 中国畜牧兽医,2011,38(7):105～107.
6 高英凯,苗永旺,苏小茜,等. 74 种鸟类线粒体基因组碱基组成及特征分析[J]. 云南农业大学学报(自然科学版),2009,24(1):51～58.
7 施燕峰. 赤麂线粒体全基因组测序及比较进化研究[D].南京:东南大学,2004.
8 Masatoshi Nei, Sudhir Kumar 著.吕宝忠,钟扬,译. 分子进化与系统发育[M].北京:高等教育出版社,2002.
9 Cui P, Ji R, Ding F, et al. A complete mitochondrial genome sequence of the wild two-humped camel(Camelus bactrianus ferus): an evolutionary history of camelidae[J].BMC Genomics,2007,18(8):241.
10 Daubin V,Perrière G. G+C3 structuring along the genome: a common feature in prokaryotes[J]. Mol Biol Evol,2003,20(4):471～483.
11 Felsenstein J. Phylogenies from molecular sequences: inference and reliability[J]. Annu Rev Genet,1988;22:521～565.
12 Kimball R T,Braun E L,Zwartjes P W,et al.A molecular phylogeny of the pheasants and partridges suggests that these lineages are not monophyletic[J].Mol Phylogenet Evol,1999,11:38～54.
13 Xu X, Wu X, Yu Z. The mitogenome of Paphia euglypta (Bivalvia: Veneridae) and comparative mitogenomic analyses of three venerids[J].Genome,2010,53(12):1041～1052.
14 Wenink P W,Baker A J,Tilanus M G. Mitochondrial control region sequences in two shorebird species: The turnstone and the dunlin,and their utility in population genetic studies[J]. Mol Biol Evol,1994,11(1) :22～31.

[1]	LI Ying, HE Guoge, WANG Yan, HE Jingyi, HUANG Aizhen, ZHENG Jingcheng, GE Yingying, LUO Chenglong. A Genetic Diversity Analysis of Mitochondrial DNA D-loop Region in Four High Quality Chicken Breeds [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(2): 469-478.
[2]	WANG Kun, DOU Tengfei, LI Qihua, LIU Mingzheng, LI Fengyun, JIA Junjing, GE Changrong. Genetic Diversity Analysis of Three Newly Discovered Native Chicken Breeds in Yunnan Using mtDNA D-loop Region Sequences [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(10): 3232-3241.
[3]	LI Xingcai, PAN Chengyong, LI Hui, YANG Huating, CHEN Lin, XIAO Tao. Study on Genetic Diversity and System Evolution of mtDNA D-Loop Region in Xianglushan Chicken [J]. China Animal Husbandry and Veterinary Medicine, 2019, 46(9): 2633-2641.
[4]	XU Bingbing, ZHANG Jiaxin, WANG Ruijun, WANG Zhiying, LI Xuewu, SONG Guoxin, ZHANG Meina, GUO Fuqiang, ZHANG Junjie, ZHANG Yanjun, SU Rui, LIU Zhihong, LI Jinquan. Research Progress on Mammalian Sperm Mitochondria [J]. China Animal Husbandry and Veterinary Medicine, 2019, 46(9): 2651-2657.
[5]	HU Hui-yan, JIA Qing, LIU Jin, HOU Sheng-kui, ZHAO Si-si, ZHANG Wei-feng. Genetic Diversity and Phylogenetic Relationships of the Complete Mitochondrial Genome in Chinese Indigenous Pig Breeds [J]. , 2017, 44(8): 2360-2368.
[6]	ZHANG Bing, LIAO Hai-hong, NONG Su-qun, MA Qing-yan, SUN Jun-li. The Genetic Diversity and Phylogenetic Relationships of Five Guangxi Pig Breeds and Three Exotic Pig Breeds by Analysis of mtDNA D-loop Sequences [J]. , 2015, 42(7): 1807-1815.
[7]	YU Ping, CAO Ting, SHI Li-guang, LIU Ya-fang, ZHAO Chun-ping, ZHANG Li-ling, HOU Guan-yu. Complete Mitochondrial DNA D-Loop Sequence and Genetic Diversity of Wuzhishan Pig [J]. , 2015, 42(5): 1222-1231.
[8]	ZHANG Xue-dong, LI Qing-hai, TONG Xun-quan, WANG Huan-huan, LOU Li-feng. Analysis on Mitochondrial DNA Control Region Polymorphism in Six Domestic Pig Breeds in Zhejiang Province [J]. , 2015, 42(2): 443-451.
[9]	HAO Gui-ying. Sequencing and Phylogenetic Analysis of Mitochondrial cox1 and nad1 Genes for Cysticercus Cellulosae in Xichang [J]. , 2015, 42(1): 61-66.
[10]	YANG Yan-chuan, LI Zuo-lei, ZHANG Nian-zhang, LIU Guang-xue, ZHU Xing-quan, SUN Xiao-lin. Sequence Variation in Mitochondrial nad5 Gene between Syphacia obvelate and Aspiculuris tetraptera [J]. , 2014, 41(6): 42-45.
[11]	HAI Tang, HUANG Jin-long, ZHAO Yi-ping, HAN Hai-ge, SA Ru-la, MANG Lai. Analysis of Genetic Diversity and Phylogeny of the Mitochondrial Cytochrome b Gene of Mongolian Horses and Mongolian Wild Horse [J]. , 2014, 41(6): 162-167.
[12]	CAI Xin, ZHAO Fang-fang, SUN Lei. Analysis of SRY Gene Polymorphisms and Patrilineal Phylogenetic Relationships for Bos grunniens and other Bos species [J]. , 2014, 41(5): 190-195.
[13]	FAN Yi-fan, LIU Guo-hua, YANG Yan-chuan, HAN Jin-huan, ZHU Xing-quan, JIANG Wen-can, SUN Xiao-lin. Cloning and Phylogenetic Analysis of Mitochondrial rrnL and nad5 Genes of Taenia pisiformis Isolates [J]. , 2013, 40(6): 45-49.
[14]	WANG Yan, YANG Yan-chuan, ZHANG Yue-wen, LI Jia-yuan, ZHU Xing-quan, LIU Guo-hua. Analysis of Sequence Variation in Mitochondrial nad4 Gene of Trichuris ovis Isolates [J]. , 2013, 40(4): 81-84.
[15]	QIAN Jian-xin, QIAN He-ping, XU Zhi-wei, YUAN Bin-bin. Phylogenetic Relationship of Bos grunniens in Qinghai Based on Mitochondrial cox1 and nad1 Genes [J]. , 2013, 40(2): 151-154.

Cloning and Sequence Analysis of Mitochondrial D-loop Gene in Chinese Hamster

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments