shRNA抑制猪繁殖与呼吸综合征病毒(PRRSV)JXA1-R株在Marc-145细胞中复制及抗PRRSV细胞株的建立

Abstract

Abstract: Porcine reproductive and respiratory syndrome (PRRS) was one of the most important infectious diseases of swine in the world.However,current vaccination strategies and antiviral drugs provided only limited protection. In this study, the ability of specific short hairpin RNA targeting to ORF1a, ORF3, ORF4, ORF5, ORF6, ORF7 were constructed, and it was found that ORF1a-4-shRNA, ORF3-1-shRNA, ORF6-2-shRNA could effectively down-regulate specific gene expression and inhibit viral replication in Marc-145 cells compared to the controls. In addition, in this study ORF6-2-shRNA was selected to be transfected into porcine fetal fibroblasts to establish anti-PRRSV cell lines. This work might provide the foundation for further research on the production of anti-PRRSV transgenetic pig.

Key words: porcine reproductive and respiratory syndrome virus (PRRSV); RNA interference; anti-PRRSV cell lines

CLC Number:

S852.4^＋3

LI Hong-mei, HE Cong, ZHANG Mao, MENG Xuan, ZHANG Jian-feng, ZHANG Jian-fei, GUO Peng-ju. Inhibition of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) JXA1-R Replication in Marc-145 Cells by shRNA and Establishment of Anti-PRRSV Cell Lines[J]. , 2013, 40(12): 12-20.

References

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Inhibition of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) JXA1-R Replication in Marc-145 Cells by shRNA and Establishment of Anti-PRRSV Cell Lines

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