猪痘病毒江西株(SWPV-JX01株)的分离鉴定

Abstract

Abstract: A virus was isolated by PK15 cells culture from skin vesicles on nursery pigs in Jiangxi province. The virus could be replicated in PK15 cells without cytopathgenic effects(CPE). The virus isolate was identified as swinepox virus (designated as SWPV-JX01) by PCR which amplified P35 gene of SWPV, and phylogenetic tree analysis based on P35 gene showed that SWPV-JX01 was 97.9% identify to reference strain(AF410153.1) of SWPV. The rabbits and Kunming mice were artificially infected by SWPV-JX01, and the P35 gene of SWPV was detected by PCR in different tissues of infected rabbits and mice. The results indicated that SWPV-JX01 could be replicated in rabbits in vivo, but could’t be replicated in mice in vivo.

Key words: swinepox virus; isolation and identification; P35 gene

CLC Number:

DENG Shun-zhou, JIANG Xin-hua, LENG Chuang, ZHANG Wen-bo. Isolation and Identification of Swinepox Virus JX01 Isolate from Jiangxi Province[J]. , 2013, 40(1): 77-81.

References

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Isolation and Identification of Swinepox Virus JX01 Isolate from Jiangxi Province

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