Abstract: Pig melanocortin-4 receptor (MC4R) gene was one of the major genes affect swine growing and finishing, to further study the biological function of the MC4R gene, preparation of high survival rate of transgenic pigs,through the use of Tet gene expression system,promoter regulatory region and transcription element of Tet-on and sequence of pig MC4R gene was amplified by PCR method. The above-mentioned components were linked into pcDNA3.1(+) to construct recombinant vector pcDNA3.1(+)-rtTA-P_Tight-MC4R by double digestions and connections methods. Restricted enzymes analysis and DNA sequencing showed that the sequence of the recombinant plasmid was correct and fragment was properly connected. Successfully constructed the Tetracycline-inducible pig MC4R eukaryotic expression vector, and in time-specific regulation of expression of the MC4R gene.

Key words: pig; MC4R gene; Tetracycline-inducible expression system