犬细小病毒病原分离及分型研究

Abstract

Abstract: To ascertain the pathogen and characteristics of 4 strains canine parvovirus, and establish foundation for further immunization research,the intestinal tract content of 4 different pathogenic dogs were inoculated into the feline kidney F81 cells after aseptic processing. We extracted the total DNA and amplified the VP2 gene with specific primers, then sequenced and typed with typical canine parvovirus’strains. 4 strains of CPV were isolated with F81 cells and the results of typing the VP2 genes showed that 4 canine parvovirus strains all belonged to CPV-2a, respectively named CPV-JQ, CPV-CM, CPV-M and CPV-KM.

Key words: canine parvovirus; isolation; VP2 gene; typing

CLC Number:

S852.65^＋5

LIANG Xiu, HU Ting-song, ZHENG Ying, FAN Quan-shui, FENG Zi-liang, CHEN Gang, QIU Wei. Isolation and VP2 Gene Typing of Canine Parvovirus[J]. , 2013, (5): 79-82.

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Isolation and VP2 Gene Typing of Canine Parvovirus

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