猪黑素皮质素受体-4基因在成纤维细胞中的沉默研究

›› 2012, Vol. 39 ›› Issue (7): 147-150.

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Study on Silencing of MC4R Gene in Pig Fibroblasts

ZHANG Dong-jie, WANG Liang, LIU Di

Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China

Revised:2011-12-18 Online:2012-07-20 Published:2012-07-16

Abstract

Abstract: MC4R, MC3R, Leptin and CCKAR are all associated with feed intake. To analysis the potential relationship of these four genes, MC4R gene was silenced in fibroblasts by RNAi method, then Real-time PCR method was used to detect the expression changes of Leptin, MC3R and CCKAR. The results showed that, after MC4R was silenced, Leptin, MC3R and MC4R expression level were not changed.

Key words: pig; feed intake; MC4R; RNAi; Real-time PCR

CLC Number:

Q78

ZHANG Dong-jie, WANG Liang, LIU Di. Study on Silencing of MC4R Gene in Pig Fibroblasts[J]. , 2012, 39(7): 147-150.

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Study on Silencing of MC4R Gene in Pig Fibroblasts

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