角蛋白26基因在超细型和细型细毛羊皮肤组织中的表达差异

Abstract

Abstract: In this study, a Real-time PCR system was developed for detection of Keratin 26 (KRT26) gene expression difference of skin tissues in Superfine and fine Merino sheep using 18S rRNA,β-actin and GAPDH genes as internal control genes based on SYBR Green Ⅰ dying technique.The results showed that threshold cycle(Ct) of the standard curve displayed good linear relationship with the concentrations of standard cDNA in a certain range.Significant difference of KRT26 expression were observed in the skin tissues of different fineness of wool sheep, and the expression ratio between super-fine and fine wool was 1.5 when the 18S rRNA,β-actin and GAPDH were reference genes respectively. It might be sure the relationship with KRT26 and the wool fineness, and this might be provide a foundation for molecular breeding and improvement of the wool traits of sheep.

Key words: KRT26; fineness; sheep; skin tissues; expression difference

CLC Number:

Q786

TIAN Yue-zhen, DI Jiang, WU Wei-wei, TIAN Ke-chuan, HUANG Xi-xia, XU Xin-ming, HA Ni-kezi稵u La-fu, FU Xue-feng, ZHANG Yan-hua, MA Yi-la稵u Er-xun, AI Mai-ti稭ai Mai-ti. Expression Difference of Keratin 26 Gene of Skin Tissues in Superfine and Fine Merino Sheep[J]. , 2012, 39(6): 31-37.

References

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Expression Difference of Keratin 26 Gene of Skin Tissues in Superfine and Fine Merino Sheep

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