高效液相色谱—紫外法对13种磺胺类药物的同步检测

›› 2012, Vol. 39 ›› Issue (4): 233-237.

• 经验交流 • Previous Articles Next Articles

Simultaneous Determination of 13 Sulphonamides by High Performance Liquid Chromatography-Ultraviolet (HPLC-UV)

FU Hong-qing^1,2, WANG Ying^2,3, ZHANG Dan², WANG Han-dong²

1. Jiangsu Animal Husbandry and Veterinary College,Taizhou 225300,China;2. College of Veterinary Medicine, Yangzhou University,Yangzhou 225009,China;3. Nantong Baby Pet Co. Ltd, Nantong 226600,China

Received:1900-01-01 Revised:1900-01-01 Online:2012-04-20 Published:2012-04-20

Abstract

Abstract: To establish a high performance liquid chromatography-ultraviolet (HPLC-UV) method for determination of several kinds of sulphonamides residues. By the analysis of the ultraviolet wavelength scanning to 13 kinds of sulfa drugs such as SD, SA, SQ, SUL, SMM, SMD, SDM', SIZ, ST, PST, SMZ, SST and ST-Na, 270 nm is used for detection wavelength. Due to the differences of pH value and structure among drugs, a variety of combinations methods were compared. Acetonitrile and acetate were used as mobile phase for gradient elution, which can separate 13 sulphonamides. There were 10 chromatographic peaks, three of ST-Na, PST and ST overlapped, and additionally two of SDM'and SMZ overlapped. The method to extract samples by acetonitrile-chloroform was proved to be good because of less interferences from impurities, over 80% recovery rate and faster analytical time.

Key words: sulphonamides; chromatography-ultraviolet; simultaneous; determination

CLC Number:

S859.79

FU Hong-qing;WANG Ying;ZHANG Dan;WANG Han-dong. Simultaneous Determination of 13 Sulphonamides by High Performance Liquid Chromatography-Ultraviolet (HPLC-UV)[J]. , 2012, 39(4): 233-237.

[1]	JIANG Bowen, LIU Guoshi, ZHANG Lu. Research Progress on the Primary Sex Determination Mechanisms in Mammals [J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(2): 614-623.
[2]	GENG Xiang, LIU Xiwang, YANG Yajun, LI Jianyong. Establishment of HPLC Method for Determination of Eprinomectin and Related Substances in Eprinomectin Sustained-release Injection [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(12): 4681-4689.
[3]	LI Xin, ZHAO Zhongli, LYU Yang, LIU Jianqiang, WEI Tian, JIN Haiguo, YU Yongsheng. Study on Differentiation of Chicken Embryonic Stem Cells to Male Germ Cells by RA [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(7): 2142-2149.
[4]	YANG Hongzao, LI Jingyu, WANG Dongsheng, ZHANG Shidong, DONG Shuwei, YAN Baoqi, NA Lidong, WU Chunli, DENG Jun, WU Guanlian, CHEN Xinli, ZHAO Liutao, ZHU Kai, LIANG Yongxi, YAN Zuoting. Determination of Patchouli Alcohol in Maxiangling Oral Liquid by GC [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(7): 2264-2276.
[5]	ZHANG Jingju, LIU Jing, XU Fei. Research Progress on Resistance of Salmonella Kentucky to Fluoroquinolones and Carbapenems [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(11): 3739-3748.
[6]	LI Haihua, CHEN Zhiqiang, CAI Xiaoli, FU Liyun, GUO Weibing, ZHANG Pengju. Establishment a Chinese Medicine Quality Standard of Qinhong Perfusate for the Treatment of Mastitis [J]. China Animal Husbandry and Veterinary Medicine, 2019, 46(12): 3775-3784.
[7]	ZOU Yi, ZHANG Xiaofeng, LIU Songbo, PENG Yunzhi, GUO Yuming, TAN Huize. Research Advances of Net Energy Application for Broiler Production [J]. China Animal Husbandry and Veterinary Medicine, 2019, 46(11): 3270-3276.
[8]	ZHU Hong-mei, LIU Cui, ZHANG Qian, ZHENG Qiu-yan, JIA Yu-jie, LIU Tao. Study on Quality Standard of Ruxiankang Injection [J]. , 2017, 44(8): 2465-2474.
[9]	LI Lei-ting, LIU Jun-hua, LIU Bao-tao, FU Hong-lei, ZOU Ming. Residue Analysis of Five Kinds of Penicillins in Eggs by High Performance Liquid Chromatography-Ultraviolet Detection [J]. , 2017, 44(8): 2483-2488.
[10]	ZHANG Li, SHAN Da-cong, LIU Yan, PAN Yu-hua. Study on Quick Sex Identification Method in Pigeons by CHD Gene [J]. , 2016, 43(5): 1379-1384.
[11]	PAN Chun-hui, YANG Hong-yu, ZHANG Xuan, REN Yuan-qin, PENG Xiao-feng, LIU Tao. Research on the Promotion of Qingwenbaidu Powder Quality Standard [J]. , 2016, 43(12): 3170-3176.
[12]	YANG Hai-feng, LI Yong-jun, JIN Li-qin, ZHAO Shan-shan, SUN Ai-rong, QIAO Xin-yue. Determination and Stablity Test of Praziquantel Injection [J]. , 2015, 42(11): 3010-3015.
[13]	Ezizjan Nabi, Shamshidin Abduriyim, Buzohra Tursun, Zilajigvl Ekram, Aynur Abduhini, Guzalnur Zibibilla, Rahmutulla Abdukerim, Mahmut Halik. Study on Determining Sex of Cervus elaphus songaricus by Using of Pellet Morphometry [J]. , 2014, 41(9): 189-193.
[14]	LIU Hong-mei,CHEN Yu-ting,HE Li-mei,PENG Xin-yu,WU Jin-jie,YUAN Ming-gui,LUO Sheng-jun,WEI Guang-wei. Determination of Total Flavonoids in Fengliao Decoction by UV-visible Spectrophotometry [J]. , 2014, 41(6): 140-144.
[15]	ZENG Jiang-yong. Sequence Determination of Full Genome and Analysis of the Gene Characteristics of Hankou/99 Strain of Foot-and-mouth Disease Virus [J]. , 2014, 41(12): 74-80.

Simultaneous Determination of 13 Sulphonamides by High Performance Liquid Chromatography-Ultraviolet (HPLC-UV)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments