GC法测定马香苓口服液中百秋李醇含量研究

doi:10.16431/j.cnki.1671-7236.2020.07.033

Abstract

Abstract: The purpose of the experiment was to establish a capillary gas chromatography (GC) method for the content determination of patchouli alcohol in Maxiangling oral liquid.The samples were determined by capillary GC.The chromatographic conditions were as follows:The chromatographic column HP-5 capillary column was used,and the column temperature was controlled by programming (the initial temperature was 150 ℃,held for 18 min,and then rose at a rate of 50 ℃/min up to 280 ℃ for 5 min).The injector temperature was 280 ℃.Carrier gas was high purity nitrogen.The flow rate was 1 mL/min.The injection volume was 1 μL,and the split ratio was 20:1.FID detector was used and the detector temperature was 280 ℃.Hydrogen flow rate was 40 mL/min and air flow rate was 370 mL/min.The system applicability test,specificity test,linear range test,determination of detection limit and quantitative limit,precision test,stability test,repeatability test,sample recovery test,durability test and content determination of ten batches of samples were conducted.The results showed that the solvent peak,impurity peak and main component peak in the specific solution could be separated effectively and the resolution of the chromatographic method met the requirements (R ≥ 1.5),which showed that the method had good applicability.No outstanding peak was detected in the negative control solution,indicating that other components in the sample did not interfere with the determination.Limit of quantitation(LOQ) and limit of detection(LOD) of patchouli alcohol were 2.842 and 0.812 μg/mL.Under the above chromatographic conditions,a good linearity was obtained in the range of 15.86-1 015 μg/mL (y=0.869x-10.45,R²=0.999,n=7);RSDs of precision,stability(intra-day precision and daytime precision) and reproducibility tests were 0.90%,1.63%and 1.83%,2.90%,respectively.RSDs were within controllable range (RSD ≤ 3%).The average recovery was 95.36%,with a RSD of 2.82%(n=6).The results showed that the established gas chromatography method had a good recovery rate for the determination of patchouli alcohol.The durability test was carried out to verify that the established chromatographic method could stably determine the content of patchouli alcohol.The methodological verification showed that the GC method was accurate,stable,convenient and feasible,and could be used as a method for the content control of patchouli alcohol in Maxiangling oral liquid.At the same time,it also provided an effective detection method for the quality control of this preparation.It was tentatively determined that the content of patchouli alcohol in this product was not less than 47.53 μg/mL.

Key words: Maxiangling oral liquid; GC; Pogostemon cablin; patchouli alcohol; content determination

CLC Number:

S859.3

YANG Hongzao, LI Jingyu, WANG Dongsheng, ZHANG Shidong, DONG Shuwei, YAN Baoqi, NA Lidong, WU Chunli, DENG Jun, WU Guanlian, CHEN Xinli, ZHAO Liutao, ZHU Kai, LIANG Yongxi, YAN Zuoting. Determination of Patchouli Alcohol in Maxiangling Oral Liquid by GC[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(7): 2264-2276.

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Determination of Patchouli Alcohol in Maxiangling Oral Liquid by GC

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