清瘟败毒散的质量标准提升研究

doi:10.16431/j.cnki.1671-7236.2016.12.013

Abstract

Abstract:

To improve the quality standard of Qingwenbaidu powder, TLC was used to qualitative identity of Coptis, Radix Rehmanniae,Gardenia, Forsythia, Rhizoma Anemarrhena and Lophatherum gracile Brongn in Qingwenbaidu powder.The content of gardenin was determined by HPLC. Stationary phase were performed on ODS C18 column (4.6 mm ID×250 mm,5 μm) and the mobile phase was acetonitrile and water (13:87),the detection wavelength was 238 nm. The results showed that in the relative position of the chromatography of the control medicinal material, the chromatogram of the test sample showed the same color spots, and the negative sample had no interference. There was a good linear relationship (R²=0.9999) when the garden in range of 0.062 to 0.370 μg. Its regression equation was y=1 789.1x-14.296 and R²=0.99984. The average recovery was 99.60% and RSD was 0.76%. This method was simple, accurate and reproducible, which could better control the quality of the Qingwenbaidu powder, and could be used as the quality standard of Qingwenbaidu powder.

Key words: Qingwenbaidu powder; quality standard; TLC; determination

CLC Number:

S854.5

PAN Chun-hui, YANG Hong-yu, ZHANG Xuan, REN Yuan-qin, PENG Xiao-feng, LIU Tao. Research on the Promotion of Qingwenbaidu Powder Quality Standard[J]. , 2016, 43(12): 3170-3176.

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Research on the Promotion of Qingwenbaidu Powder Quality Standard

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