肿节风三清颗粒的薄层色谱鉴别和含量测定

doi:10.16431/j.cnki.1671-7236.2019.10.032

Abstract

Abstract: This study was aimed to establish a method for the identification and content determination of Zhongjiefeng Sanqing granule,so as to provide a methodological basis for the quality standards of the preparation.The Zhongjiefeng,Shegan and Gancao in Zhongjiefeng Sanqing granule were qualitatively analyzed by thin layer chromatography (TLC);The content of isofraxidin and rosmarinic acid in the preparation was determined by high performance liquid chromatography (HPLC).The results of TLC showed that Zhongjiefeng,Shegan and Gancao in Zhongjiefeng Sanqing granule had the same fluorescent spots at the same position as the corresponding standard medicinal materials or standards,while the negative samples had no characteristic fluorescent spots.The HPLC results showed that the linear relationship of isofraxidin in the range of 3.795 to 18.975 μg/mL and rosmarinic acid in the range of 6.035 to 30.175 μg/mL were both good,and the R² value were 0.9995 and 0.9997,respectively.The average recoveries of isofraxidin and rosmarinic acid were 99.13% and 100.30%,respectively;And the RSD was 1.98% and 0.02% (n=6),respectively.The results showed that the established identification and quantitative analysis methods were operability,accurate and reliable,and could be used for the identification of Zhongjiefeng,Shegan,Gancao and the determination of the components of isofraxidin and rosmarinic acid in Zhongjiefeng Sanqing granule,which was important to provide a methodological basis for establishment of the quality standard of Zhongjiefeng Sanqing granule.

Key words: Zhongjiefeng Sanqing granule; isofraxidin; rosmarinic acid; quality standard; thin layer chromatography; high performance liquid chromatography

CLC Number:

S853.7

DENG Ruihan, LIN Bailiang, YANG Liu, LU Yanrong, PENG Jianbo, WU Wenjian, YANG Lei, HAO Zhihui, YANG Shanzhong, HE Jiakang. Thin Layer Chromatography Identification and Content Determination of Zhongjiefeng Sanqing Granule[J]. China Animal Husbandry and Veterinary Medicine, 2019, 46(10): 3095-3105.

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Thin Layer Chromatography Identification and Content Determination of Zhongjiefeng Sanqing Granule

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