治疗乳房炎的中药芩红灌注剂质量标准的建立

doi:10.16431/j.cnki.1671-7236.2019.12.038

Abstract

Abstract: Qinhong perfusate is a newly developed Chinese medicine compound preparation for the treatment of dairy cow mastitis.In order to ensure the safety of clinical medication and the stability of production technology,the quality standard of Qinhong perfusate was studied and established.Scutellaria baicalensis,honeysuckle and safflower were identified by TLC,referring to the inspection methods of perfusate in Chinese Veterinary pharmacopoeia.The relative density,pH,sterility,related substances and contents of Qinhong perfusate were examined,and baicalin was quantitatively detected by high performance liquid chromatography.The results showed that the samples of Qinhong perfusate showed the same color spots at the corresponding positions with the control medicinal materials and the control products.The TCL method for identification of Scutellaria baicalensis,honeysuckle and safflower had good specificity,reproducibility and durability.The relative density,pH,sterility,related substances and loading amount of Qinhong perfusate all conformed to the relevant regulations,and the relative density of Qinhong perfusate was not less than 1.0 (30 ℃) and the pH was 5.0-7.0.The precision,stability,repeatability,linearity,specificity and durability of the HPLC method for determination of baicalin content in Qinhong perfusate were good.The linear relationship between baicalin content and peak area was good in the range of 0-9.20 μg(R²=0.9999).The average recovery was 100.04%(RSD=1.34%,n=9).The established quality standard was accurate,feasible and reproducible.It could be used for the quality control of Qinhong perfusate and laid a foundation for its large-scale production and clinical application.

Key words: Qinhong perfusate; quality standard; mastitis; TLC; determination of content; baicalin

CLC Number:

S853.7

LI Haihua, CHEN Zhiqiang, CAI Xiaoli, FU Liyun, GUO Weibing, ZHANG Pengju. Establishment a Chinese Medicine Quality Standard of Qinhong Perfusate for the Treatment of Mastitis[J]. China Animal Husbandry and Veterinary Medicine, 2019, 46(12): 3775-3784.

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Establishment a Chinese Medicine Quality Standard of Qinhong Perfusate for the Treatment of Mastitis

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