China Animal Husbandry & Veterinary Medicine ›› 2012, Vol. 39 ›› Issue (3): 81-84.

• 生物技术 • Previous Articles     Next Articles

Brief Introduction of Immunefluorescent Test Technology and Application Progress on Parasites Detection

WANG Xiao-huan1,2, YANG Lian-ru1, ZHAO Lin-li3, WU Shao-qiang2   

  1. 1. College of Veterinary Science,Inner Mongolia Agriculture University,Huhhot 010018,China;2. Chinese Academy of Inspection & Quarantine,Beijing 100029,China;3. Inner Mongolia Entry and Exit Inspection and Quarantine,Huhhot 010020,China
  • Received:1900-01-01 Revised:2011-12-30 Online:2012-03-20 Published:2012-03-20

Abstract: Immunofluorescence technique is a new detection technology which is based on biochemistry,microscopy and immunology. Its basic principle is using fluorescence-labeled antibody or antigen to combine with the antigen or antibody in the samples,and the fluorescence can be recognized under a microscope. The microscopic accuracy and the immunological specificity and sensitivity can be organically combined in this method. As a rapid diagnostic method,immunofluorescence technique is widely used in medical and biological research. It is a general method in the diagnosis of virus,bacterium or parasite caused diseases,such as Toxoplasma gondii infection. With the development of labeled antigen and antibody,immunofluorescence technique will be widely used in parasitosis diagnosis.

Key words: immunofluorescence technique; parasite; detection