Abstract: The gene of prion protein protease resistance fragment PrP^res of the Sika deer was amplified by PCR using a pair of specific primers. The product of PCR was cloned into the expression vectors of pET-Trx and pET-His. The restructuring plasmids were transformed into the host bacterium of E.coli BL21 (DE3) plys, at 37 ℃ for 4 h. SDS-PAGE result illustrated that fusion proteins of Trx-PrP^res and His-PrP^res were highly expressed. The expression level of fusion proteins were 38.2% and 30.1%, respectively.

Key words: Sika deer; prion protein; PrP^sc ; proteinase resistant protein; prokaryotic expression