禽流感病毒WUDI-H9N2株的分离鉴定及 HA基因的分子特征研究

Abstract

Abstract: An isolate of avian influenza virus,designated WUDI-H9N2,was isolated from Shandong area of China in Auguste 2011 and identified for pathogenicity to chicken embryo by hemagglutination test, hemagglutination inhibition test and RT-PCR. A pair of primers was designed to amplify the HA strain based on the reported gene sequences in GenBank by Oligo 6.0 and for WUDI-H9N2 HA gene amplifying and sequence analysis. The separated AIV H9 strain and other serotype were respectively analysed by nucleotide and amino acid phylogeny tree. The results of sequencing showed that the HA gene was 1708 bp in legth, no nucleotide insertion and deletion, the cDNA contains whole open reading frame(ORF) of HA gene was 1683 bp and encoded a 560 amino acid protein. Compared with reference strains, the nucleotide sequence similarity of HA was 92.9% to 98.1%. On the basis of phylogenetic analysis of HA gene, we concluded that the HA shared 98.1% similarity of structure protein encoding sequence with A/chicken/China/AH-10-012010(H9N2) strain.

Key words: avian influenza virus; HA gene sequence; open reading frame; phylogenetic analysis

CLC Number:

XIE Yin-lu, ZHANG Yu, DIAO Fu-hua. Isolated and Identified of Avian Influenza Virus WUDI-H9N2 in China and Molecular Characteristics Research of HA Gene[J]. , 2012, 39(10): 87-90.

References

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Isolated and Identified of Avian Influenza Virus WUDI-H9N2 in China and Molecular Characteristics Research of HA Gene

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