禽流感病毒WUDI-H9N2株的分离鉴定及 HA基因的分子特征研究

Abstract

Abstract: An isolate of avian influenza virus,designated WUDI-H9N2,was isolated from Shandong area of China in Auguste 2011 and identified for pathogenicity to chicken embryo by hemagglutination test, hemagglutination inhibition test and RT-PCR. A pair of primers was designed to amplify the HA strain based on the reported gene sequences in GenBank by Oligo 6.0 and for WUDI-H9N2 HA gene amplifying and sequence analysis. The separated AIV H9 strain and other serotype were respectively analysed by nucleotide and amino acid phylogeny tree. The results of sequencing showed that the HA gene was 1708 bp in legth, no nucleotide insertion and deletion, the cDNA contains whole open reading frame(ORF) of HA gene was 1683 bp and encoded a 560 amino acid protein. Compared with reference strains, the nucleotide sequence similarity of HA was 92.9% to 98.1%. On the basis of phylogenetic analysis of HA gene, we concluded that the HA shared 98.1% similarity of structure protein encoding sequence with A/chicken/China/AH-10-012010(H9N2) strain.

Key words: avian influenza virus; HA gene sequence; open reading frame; phylogenetic analysis

CLC Number:

XIE Yin-lu, ZHANG Yu, DIAO Fu-hua. Isolated and Identified of Avian Influenza Virus WUDI-H9N2 in China and Molecular Characteristics Research of HA Gene[J]. China Animal Husbandry & Veterinary Medicine, 2012, 39(10): 87-90.

References

1 杜景娇,薛强,邹明强,等.禽流感病毒检测技术的研究新进展[J].中国畜牧兽医,2012,39(5):232~236.
2 唐秀英,付朝阳,冯菊艳. H9亚型禽流感的流行与防制[J].预防兽医学进展,2002,2(4):1~3.
3 黄庚明,辛朝安. 禽流感病毒血凝素(HA)基因的研究进展[J].广东畜牧兽医科技,2001,26(2):3~5.
4 Butt K M, Smith J D, Chen H, et al. Human infection with an avian H9N2 influenza a virus in HongKong in 2003[J]. Journal of Clinical Microblogy, 2005, 43(11):5760~5767.
5 Capua I,Alexander D J. Avian influenza and human health[J]. Acta Trop, 2002,83(1):1~6.
6 Chen H, Matsuoka Y, Chen Q, et al. Generation and characterization of a cold-adapted influenza a H9N2 reassortant as a live pandemic influenza virus vaccine candidate[J]. Vaccine, 2003, 21(27~30):4430~4436.
7 Choi Y K, Ozaki R J H,Webster R J, et al. Continuing evolution of H9N2 influenza virus in southeastern China[J]. J Virol, 2004, 78:8609~8614.
8 Guan Y, Peiris J S, Kong K F, et al. H5N1 influenza virus isolated from geese in southeastern China: Evidence for genetic reassortment and interspecies transmission to ducks[J].Virology, 2002,292:16~23.
9 Guan Y, Shortridge K F, Scott Krauss, et al. H9N2 influenza viruses possessing H5N1-like internal genomes continue to circulate in poultry in southeastern China[J]. J Virol, 2000,74:9372~9380.
10 Li C, Yu K, Tian G, et al. Evolution of H9N2 influenza viruses from domestic poultry in mainland China[J]. Virology,2005,340 (1):70~83.
11 Lin Y P, Shaw M, Gregory V, et al. Avian-to-human transmission of H9N2 subtype influenza a virus: Relationship between H9N2 and H5N1 human isolates[J]. Proc Natl Acad Sci USA, 2000,97:9654~9658.
12 Mase M, Eto M, Imai K, et al. Characterization of H9N2 influenza a viruses isolated from chicken products imported into Japan from China[J]. Epidemiol Infect, 2006,7: 1~6.
13 Peiris J S M, Guan Y, Markwell D, et al. Cocirculation of Avian H9N2 and contemporary "Human" H3N2 influenza a virus in pigs in Southeastern China: Potential for genetic reassortment[J].Journal of Virology, 2001, 75(20):9679~9686.
14 Vey M, Orlich M, Adler S, et al. Hemagglutinin activation of pathogenic avian influenza viruses of serotype H7 requires the protease recognition motif R-X-K/R-R[J]. Virology, 1992,188(1):408~413.

[1]	CHI Zhouying, LI Tianxu, WU Yaxin, QU Xiaoyun, LI Sijie, SUN Minhua, ZHANG Jianfeng, LIAO Ming, DU Shouwen. Exploration of Respiratory Tract Response Patterns in Chickens Infected with H9N2 Subtype Avian Influenza Virus [J]. China Animal Husbandry & Veterinary Medicine, 2024, 51(9): 3970-3979.
[2]	LIAO Siyu, ZHENG Xin, DAI Qi, XU Shiyi, ZHANG Tianxu, ZHANG Xiuqiao, GUI Chun. Study on the Anti-AIV-H9N2 Activity of the Ethyl Acetate Extract from Alternanthera philoxeroides in vitro and in vivo [J]. China Animal Husbandry & Veterinary Medicine, 2024, 51(2): 759-769.
[3]	BIJunming, DONG Yaqin, CUIJin, SHA Zhou, GU Congcong, ZHENG Hui, WEI Rong, SUN Fuliang, ZHANG Feng, ZHANG Zhihong. Isolation，Identification and Genetic Evolution Analysis of a Strain of Bovine Viral Diarrhea Virus in Shandong Province [J]. China Animal Husbandry & Veterinary Medicine, 2024, 51(10): 4485-4499.
[4]	LONG Feng, XIE Shouyu, CUI Pengfei, SHI Kaichuang, WEI Xiankai, FENG Shuping, QU Sujie, LU Wenjun, LI Jianfeng, YIN Yanwen, DENG Guohua. Genetic Evolutionary Dynamics Analysis of HA and NA Genes of H9 Subtype Avian Influenza Virus in Guangxi from 2016 to 2021 [J]. China Animal Husbandry & Veterinary Medicine, 2023, 50(5): 1947-1958.
[5]	XIE Shouyu, WANG Ruimin, CUI Pengfei, SHI Kaichuang, WEI Xiankai, FENG Shuping, LONG Feng, QU Sujie, LU Wenjun, HUANG Jinshan, HUANG Fengmei, WEN Xinrui, YIN Yanwen, DENG Guohua. Molecular Characteristics of Complete Genomes of Two H3 Subtype Avian Influenza Virus Isolates Derived from Sea Duck in Guangxi Province [J]. China Animal Husbandry & Veterinary Medicine, 2023, 50(4): 1319-1328.
[6]	LI Zhongbo, HOU Qianghong, DUAN Deyong. Haplotypes Diversity,Genetic Differentiation and Phylogenetic Analysis of Aedes albopictus [J]. China Animal Husbandry & Veterinary Medicine, 2023, 50(4): 1452-1460.
[7]	JIN Huan, TU Min, SHI Aihua, ZHAO Lei, SHEN Jia, XI Shuo, ZHANG Jianwei, ZHANG Zhenhua. Isolation,Identification of H9 Subtype Avian Influenza Virus and Immune Protection Effect of Inactivated Trivalent Vaccine of Newcastle Disease,Avian Influenza (H9 Subtype),and Avian Adenovirus Disease Against the Viruses [J]. China Animal Husbandry & Veterinary Medicine, 2023, 50(12): 5013-5021.
[8]	XIONG Chenyong, YIN Yanwen, SHI Kaichuang, LI Jun, ZHENG Min, WEI Xiankai, FENG Shuping, LONG Feng, QU Sujie, LU Wenjun, ZHOU Hongjin, HUANG Hailian, XIE Shouyu, LI Zongqiang. Genetic Diversity Analysis of Duck Tembusu Virus in Guangxi During 2019-2021 [J]. China Animal Husbandry & Veterinary Medicine, 2022, 49(8): 3099-3111.
[9]	LIU Yutong, YAO Yanting, LI Ruiling, HAN Wenqi, SHI Ting, WANG Yidan, REN Yupeng. Analysis of Genetic Variation of Porcine Epidemic Diarrhea Virus in Parts of Southwest China from 2020 to 2021 [J]. China Animal Husbandry & Veterinary Medicine, 2022, 49(6): 2279-2290.
[10]	YANG Jing, ZHANG Xinyu, LIANG Zhipeng, CHENG Qing, WANG Congying, CHI Shihong, YUAN Sheng, GUO Jinyue, HUANG Shujian, WEN Feng. Prokaryotic Expression of NP Protein of H9N2 Subtype Avian Influenza Virus and Preparation of Polyclonal Antibody [J]. China Animal Husbandry & Veterinary Medicine, 2022, 49(10): 3963-3971.
[11]	XU Minsheng, KE Haiyi, YANG Dongxia, SHI Keda, SUN Zeyi, NIU Jiawei, CHANG Xin, ZHAI Shaolun, ZANG Yingan, LI Chunling. Whole Genome Sequencing and Biological Characteristics Analysis of Multidrug-resistant Actinobacillus pleuropneumoniae GD2107 Strain [J]. China Animal Husbandry & Veterinary Medicine, 2022, 49(10): 4019-4031.
[12]	QI Yuzhuo, LIANG Lin, HAN Kun, LIANG Ruiying, JIA Yaxiong, CUI Shangjin. Genome Determination and Genetic Evolution Analysis of a Pigeon Circovirus Strain in Beijing [J]. China Animal Husbandry & Veterinary Medicine, 2022, 49(1): 32-42.
[13]	JIANG Ning, YIN Hang, ZHANG Yanwei, LI Zixin, CHI Xiaojuan, WANG Song. Research Progress on Co-infection of H9N2 Subtype Avian Influenza Virus with Other Pathogens [J]. China Animal Husbandry & Veterinary Medicine, 2021, 48(9): 3447-3455.
[14]	YIN Yanwen, SHI Kaichuang, SUN Wenchao, XIE Shouyu, QU Sujie, LU Wenjun, WANG Luxia, QIN Yong, PEI Xingbiao, LING Dan. Surveillance Analysis of Low Pathogenic Avian Influenza Virus in Border Areas of Guangxi from 2013 to 2019 [J]. China Animal Husbandry & Veterinary Medicine, 2021, 48(8): 3002-3009.
[15]	XU Ning, GE Guiyang, LI Dongli, LIU Yi, GONG Qinglong, MA Baoyi, SHENG Chenyan, SHI Kun, LI Jianming, LENG Xue, DU Rui. Isolation and Identification of Porcine Pseudorabies Virus and Genetic Variation Analysis of Partial Virulence Gene [J]. China Animal Husbandry & Veterinary Medicine, 2021, 48(5): 1794-1803.

Isolated and Identified of Avian Influenza Virus WUDI-H9N2 in China and Molecular Characteristics Research of HA Gene

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments