Abstract: To amplify pig interferon-γ(pIFN-γ) cDNA from ConA-activated peripheral-blood lymphocyte by RT-PCR, then was cloned into pMD18-T and sequenced, and recombined with pET32a(+) vector, the target protein His×6 IFN-γ was induced by IPTG;the sequence analysis showed that the nucleotide sequences of the IFN-γ in the study shared 98.6% to 100.0% homology with that of pig published in GenBank, and the identity of amino acid was 96.4% to 99.4%.This recombinant protein, a molecular weight of about 27 ku and mainly presenting in inclusion bodies, was approved to have immunological activity by Western blotting analysis and SDS-PAGE. It laid a foundation for further research of IFN-γ.

Key words: gene cloning; prokaryotic expression; interferon-γ gene; pig