Abstract: Using PCR and direct sequencing to analysis the mitochondrial DNA (mtDNA) control region of Aix galericulata. Compare with reported sequence in GenBank to analysis sequence variation about three districts of mtDNA D-loop. The Aix galericulata (samples collected from Shiqian county in Guizhou province) mtDNA control region was 1035 bp. Compare with Foreign mandarin duck, there were 25 transformations, 12 transeversions, 2 insertions and 12 deletions in mtDNA control region. The mutation rate of mtDNA control region's three districts were 14. 5%, 0. 64% and 0. 8%, respectively. So the mutation rate of I region district was the fastest. The base content of chinesc Aix galcriculata mtDNA control region were A 26. 6%, C 16. 0%, T 26. 6% and G 30. 8%, A+T was slightly higher than C+G, G was the highest which was in accordance with the biased of sequence composition. Compare with other Anatidac specics,the conforming were all higher than 80%.

Key words: Aix galcriculata; mitochondrial DNA(mtDNA)control region; scqucncc; variation