Abstract: In this experiment,the system and condition of nested PCR to detect MG were established with two pairs of primers which had been designed by the predecessors according to the adherin protein pvpA gene sequence of MG published in GenBank.Theremore, the sensitivity and specificity of this method was analysed.The results showed that this method could detect more than 0.18 pg/μL MG DNA.However, based on these two pairs of internal and external primers, the PCR did not amplify the specific fragment with the template DNA from common bacterium and virus of chicken. The results showed that this PCR amplification system was specific and sensitive. It can be used to detect early stages of MG infection in clinical diagnosis.

Key words: MG; nested PCR; adherin protein-PvpA; detection