猪Mx1基因真核表达载体的构建、鉴定及其表达

Abstract

Abstract: The purpose of this study was to obtain pig fibroblast cells that transferred Mx1 gene. Total RNA was extracted from pig fibroblast cells that induced with IFN and the cDNA encoding pig Mx1 protein was cloned using reverse transcription polymerase chain reaction (RT-PCR). The Mx1 gene was subcloned into pMSCV-IRES-GFP vector and then the recombinant plasmids were transferred into Luchuan pig fibroblast cells by the method of Lipofectaming 2000. The results showed that Mx1 gene had been integrated into the genome of Luchuan pig fibroblast cells based on the analysis of fluoroscopy and PCR identification.

Key words: fibroblast cells; Mx1 gene; Luchuan pig

CLC Number:

QIN Zhao-xian;PAN Tian-biao;HUANG Guang-yun;XIE Bing-kun. Construction, Identification and Expression of Eukaryotic Expression Vector of Pig Mx1 Gene[J]. , 2011, 38(4): 95-97.

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Construction, Identification and Expression of Eukaryotic Expression Vector of Pig Mx1 Gene

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