基于ISSR分子标记分析陆川猪种群遗传差异

doi:10.16431/j.cnki.1671-7236.2019.12.019

Abstract

Abstract: This study was aimed to investigate the genetic differences and genetic diversity in Luchuan pig populations,provide reference for germplasm improvement and innovation.A total of 29 samples of Luchuan pigs and some samples of Guangxi local pigs were used as experimental materials,three bands of clear and reproducible ISSR primers were used,and DNA fragments obtained by PCR amplification and electrophoresis were used to carry out cluster analysis and clustering trees were constructed to analyze the genetic differences in Luchuan pig populations.The results showed that a total of 182 bands were found on the ISSR molecular markers,84 of which were polymorphic.The total DNA fragment bands amplified by three ISSR primers were different from the polymorphic DNA fragments,which indicated that the selected three ISSR molecular markers were abundant in Luchuan pigs populations.The genetic similarity coefficient in Luchuan pig populations rangeed from 0.43 to 1.00,which indicated that the genetic differences in populations were larger,and the genetic background was rich.UPGMA cluster analysis results showed that Luchuan pigs and Chang Lu binary pigs,Bama Xiang pigs and Huanjiang Xiang pigs could be grouped together,and Da Lu binary pigs,ternary pigs and Longbao pigs were grouped together.This study confirmed that the selected three ISSR primers could be used as effective genetic markers for analyzing genetic difference in Luchuan pig populations,enriched the current ISSR molecular marker resources of Luchuan pigs,and also indicated the rich genetic diversity in Luchuan pig populations.

Key words: Luchuan pigs; ISSR; molecular marker technique; genetic similarity coefficient; cluster analysis

CLC Number:

S813.3

LI Zhongyu, FENG Yi, HUANG Li, WANG Miao, TENG Jianwen. Genetic Differences Analysis in Luchuan Pigs Populations Using ISSR Molecular Marker[J]. China Animal Husbandry and Veterinary Medicine, 2019, 46(12): 3617-3626.

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Genetic Differences Analysis in Luchuan Pigs Populations Using ISSR Molecular Marker

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