Abstract: The coding region of human genomic genes of the IL-3,GM-CSF and lysozyme (Hly) were obtained by PCR method. The IL-3 and GM-CSF genes were linked with the pMD19-T vector, and the Hly gene was linked with the pEASY-Blunt vector. Then the eukaryotes expression vector for these genes were constructed by insert these gene fragments into pIRESneo vector. Following, transfection the PK15 cells with these eukaryotes expression vector respectively. The total RNA of the transfected cells were extracted after 48 hours and the cDNA of the human genes of the IL-3,GM-CSF and Hly were obtained by RT-PCR method. The results showed that the sequences were completely homologous with the registered ones of the NCBI.

Key words: IL-3 gene; GM-CSF gene; Hly gene; cDNA; cloning; sequencing