广西陆川猪猪肺炎支原体P97基因R1区克隆及序列分析

doi:10.16431/j.cnki.1671-7236.2015.09.008

Abstract

Abstract: Through in-depth understanding of sequence characteristics, structure and genetic variation of adhesion factor P97 gene R1 region of Mycoplasma hyopneumoniae (Mhp) epidemic strains in Guangxi Luchuan pig, the assay was aimed to provide theoretical basis for mycoplasmal pneumonia of swine (MPS) integrated effective prevention and control measures of Guangxi local variety Luchuan pig, and lay a solid foundation for further study the characteristics of Mhp epidemic strains in Guangxi Luchuan pig.A pair of primers was designed to amplify P97 gene R1 region according to Mhp genome sequence, the MPS positive disease materials of Guangxi Luchuan pig from 2011 to 2014 as the object of study, after genomic DNA extraction and PCR amplification of P97 gene R1 region, the PCR products were sequenced.The base composition and deduced amino acid sequence of P97 gene R1 region of Mhp epidemic strains of Guangxi Luchuan pig were compared.The analysis of DNAStar showed that there were multiple base mutations in P97 gene R1 region of 15 strains of Guangxi Luchuan pig Mhp strains (GXLC-1, GXLC-2, GXLC-3, GXLC-4, GXLC-5, GXLC-6, GXLC-7, GXLC-8, GXLC-9, GXLC-10, GXLC-11, GXLC-12, GXLC-13, GXLC-14 and GXLC-15) and 3 strains of Guangxi other breeds of pigs Mhp strains (GX227, GX595 and GX674).The statistical repeat number of five amino acid (AAKPV/E) of P97 R1 region were 9 to 18, the average value was 12, TN repeats were 1 to 4.We found that Guangxi Luchuan pig Mhp strains mutation made its virulence and adhesion ability enhancement, and respiratory of Luchuan pig with the special structure of short and narrow, which could more easily lead to the occurrence of Guangxi Luchuan pig MPS.

Key words: Guangxi Luchuan pig; Mycoplasma hyopneumoniae; P97 gene R1 region; clone; sequence analysis

CLC Number:

LI Ke-yu, ZHAO Wu, LI Bin, QIN Yi-bin, LU Bing-xia, LIANG Jia-xing, HE Ying, ZHOU Ying-ning, WEI Ying-yi. Cloning and Sequence Analysis of P97 Gene R1 Region of Mycoplasma hyopneumoniae in Guangxi Luchuan Pig[J]. , 2015, 42(9): 2270-2277.

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Cloning and Sequence Analysis of P97 Gene R1 Region of Mycoplasma hyopneumoniae in Guangxi Luchuan Pig

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