猪伪狂犬病病毒NP株gE、gI基因的克隆及序列分析

doi:10.16431/j.cnki.1671-7236.2015.09.007

Abstract

Abstract: According to published gE and gI gene sequences of pseudorabies virus (PRV) in GenBank, we designed two pairs of primers for PCR amplification of gE and gI genes of PRV NP isolate, after PCR products recycling, cloning and sequencing, the sequencing results were consistent with expectations of PRV gE and gI genes.Homology comparison analysis results revealed that compared with the domestic PRV strains, the homologies of gE and gI amino acids of PRV NP isolate were 95.7% to 99.8% and 89.9% to 99.5%, respectively.Phyogenetic tree analysis and amino acid sequence alignment results found that the gE amino acid sequence site changes of PRV NP isolate were the same with the PRV strains which were isolated from domestic in 2012, thus we could speculate that PRV NP isolate had mutanted.This study had laid the foundation for the epidemiological investigation and analysis of PRV, also provided the scientific basis for the development of scientific, effective and new pseudorabies vaccine.

Key words: pseudorabies virus (PRV); gE gene; gI gene; sequence analysis

CLC Number:

LIN Qun-qun, ZHENG Xiao-xiang, CHEN Peng-qiang, CHEN Qiu-yong, ZENG Xian-cheng, HU Chong-wei, XIU Jin-sheng. Cloning and Sequence Analysis of gE and gI Genes of Pseudorabies Virus NP Isolate[J]. , 2015, 42(9): 2262-2269.

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Cloning and Sequence Analysis of gE and gI Genes of Pseudorabies Virus NP Isolate

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