陆川猪脂酰辅酶A氧化酶1基因克隆及序列分析

doi:10.16431/j.cnki.1671-7236.2017.03.002

Abstract

Abstract:

The objective of this study was to clone the acyl-coenzyme A oxidase 1 (ACOX1) gene of Luchuan pig and analyze its genetic structure with bioinformatics. A pair of special primer was designed according to predicted sequence of pig ACOX1 gene in GenBank. The ACOX1 gene was amplified by RT-PCR, the physicochemical property and secondary structure of ACOX1 gene were systemically analyzed by bioinformatics techniques. The results showed that ACOX1 gene fragment included an 1 986 bp whole length CDS (coding 661 bp amino acids). The sequence multi-aligned results showed that Luchuan pig shared 99.5%, 85.5%,87.1%,66.3%,75.6%,84.0%,82.3%,81.1% and 69.1% of similar nucleotide sequence with that of pig,human, zebrafish, chicken, rhesus monkeys, mice, rat and frog, respectively. The prediction of ACOX1 secondary structure showed that Luchuan pig ACOX1 had no signal peptide and transmembrane structure. This study suggested that the whole CDS sequence of ACOX1 gene was successfully cloned in Luchuan pig, and the cloning and analysis of ACOX1 gene provided an important foundation for further studying on the fatty deposition and lipornetabolism of ACOX1 gene in Luchuan pig.

Key words: Luchuan pig; ACOX1 gene; cloning

CLC Number:

Q785

GUAN Zhi-hui, CHEN Bao-jian, PAN Tian-biao, QIN Zhao-xian, LIU Jia-qi, MA Qing-yan, NONG Su-qun, XIE Bing-kun. Cloning and Sequence Analysis of ACOX1 Gene in Luchuan Pig[J]. , 2017, 44(3): 628-634.

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Cloning and Sequence Analysis of ACOX1 Gene in Luchuan Pig

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