牛结核杆菌DNA疫苗的纯化工艺研究及质量鉴定

›› 2011, Vol. 38 ›› Issue (4): 186-190.

• 疾病防治 • Previous Articles Next Articles

Study the Preparation Technology and Quality Evaluation of Mycobacterium Bovis DNA Vaccine

SONG Zhen-zhong¹, HUANG Jiong¹, XU Xue-ping², XU Zhi-guang¹, ZHANG Yun-feng², CAI Hong³, XU Li-yi¹, HU Xi-dan¹

1. Institute of Veterinary,Xinjiang Academy of Animal Science,Urumqi 830000,China;2. Institute of Animal Husbandry and Veterinary Medicine,Xinjiang Academy of Agricultural and Reclamation Sciences,Shihezi 832000,China;3. College of Life Sciences, Peking University, Beijing 100871, China

Received:2010-09-28 Revised:1900-01-01 Online:2011-04-20 Published:2011-04-20

Abstract

Abstract: In order to get high purity plasmids DNA which can be used for animal immunity,on the base of high density fermentive engineering bacteria,plasmids was isolated rudely through conventional alkaline lysis. Major RNA in the alkaline lysate was removed by calcium chloridize,and clarified solution was obtained by Sartobran P bladder-type filter; plasmid supercoil DNA was precipitated,and major bacteria proteins and host genomic DNA were removed by polyethyene glycol method. Finally,the quality monitoring of the DNA vaccine was accomplished the requirement for transfection of animal.This technique achieved one-step chromatographic purification of DNA vaccine,with the advantages of simplicity and rapidity.

Key words: DNA vaccine; purification; PEG 8000; AIEX; quality evaluation

CLC Number:

SONG Zhen-zhong;HUANG Jiong;XU Xue-ping;XU Zhi-guang;ZHANG Yun-feng;CAI Hong;XU Li-yi;HU Xi-dan. Study the Preparation Technology and Quality Evaluation of Mycobacterium Bovis DNA Vaccine[J]. , 2011, 38(4): 186-190.

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Study the Preparation Technology and Quality Evaluation of Mycobacterium Bovis DNA Vaccine

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