应用固相竞争ELISA、液相阻断ELISA和中和试验检测口蹄疫抗体的比较研究

›› 2011, Vol. 38 ›› Issue (4): 183-185.

• 疾病防治 • Previous Articles Next Articles

Comparative Study between New-established FMD Antibody Identification Method SPC-ELISA and Traditional Methods LPB-ELISA and VNT

LIAO De-fang¹, LI Le¹, MIAO Hai-sheng¹, GAO Lin¹, XIN Ai-guo¹, ZHU Ming-wang², LI Hua-chun¹

1. Yunnan Animal Science and Veterinary Institute,Kunming 650224,China;2. Yunnan Baoshan Vaccine Plant,Baoshan 678000,China

Received:1900-01-01 Revised:2011-01-10 Online:2011-04-20 Published:2011-04-20

Abstract

Abstract: This study was designed to evaluate the correlation between new-established FMD antibody identification method SPC-ELISA and traditional methods LPB-ELISA and VNT. In this study,94 serum samples (10 from swine,8 from bovine and 76 from yak) which were collected from the field were used to do SPC-ELISA,LPB-ELISA and VNT to detect FMD antibody. Coincidence rate and positive rate were calculated after experiments. The result indicated that,new-established SPC-ELISA had very high coincidence rate with VNT,which was 88.3%. Swine and yak serum’s coincidence rate with VNT were even higher,respectively 90% and 89.5%. Besides,SPC-ELISA also had a higher positive rate than LPB-ELISA,which was more relevance to the positive rate of VNT. The conclusion of the analysis showed new-established FMD antibody detection method SPC-ELISA had greater superiority than traditional method LPB-ELISA.

Key words: FMD; SPC-ELISA; LPB-ELISA; VNT; coincidence rate; positive rate

CLC Number:

S852.4

LIAO De-fang;LI Le;MIAO Hai-sheng;GAO Lin;XIN Ai-guo;ZHU Ming-wang;LI Hua-chun. Comparative Study between New-established FMD Antibody Identification Method SPC-ELISA and Traditional Methods LPB-ELISA and VNT[J]. , 2011, 38(4): 183-185.

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Comparative Study between New-established FMD Antibody Identification Method SPC-ELISA and Traditional Methods LPB-ELISA and VNT

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