Abstract: In order to establish a rapid and sensitive method to detect E.wenyoni, two pairs of LAMP specific primers were designed and synthesized according to the sequence of 16S rRNA gene(AF016546) published in GenBank; loop-mediated amplification (LAMP) for detecting E.wenyoni was constructed and the reaction conditions of LAMP were optimized; sensitivity and specificity tests were carried out. LAMP results were judged by colorable reaction, electrophoretic analysis. The results showed that the LAMP product in E.wenyoni tube were confirmed by gel electrophoresis and a characteristic DNA ladder was observed. After adding SYBR Green Ⅰ to the reaction tube, green fluorescence was observed with E.wenyoni tube. The detection limit of LAMP assay was 25.6 fg/μL DNA. The specific test proved that in colorable reaction the detection tube of E.wenyoni was positive, while Eperythrozoon suis, bovine Neospora, Toxoplasma gondii, Theileria sergenti and as the comparisons were all negative. This research demonstrated that LAMP was a sensitive, specific and rapid method which was suitable for detection of E.wenyoni.

Key words: E.wenyoni; 16S rRNA gene; LAMP