›› 2010, Vol. 37 ›› Issue (7): 64-66.

• 生物技术 • Previous Articles     Next Articles

Development of PCR to Detect Porcine Circovirus Type 2 and its Clinical Application

LIU Jian1, CAO Huo-ren2, ZHOU Jin-ping1, JU Hou-bin1, ZHANG Wei-yi1，
WANG Jian1, LIU Pei-hong1   

  1. (1.Shanghai Center for Animal Disease Control and Prevention, Shanghai 201103, China;2.Shanghai Songjiang Center for Animal Disease Control and Prevention, Shanghai 201611, China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-07-20 Published:2010-07-20

Abstract: As porcine circovirus type 2 (PCV-2) is an important pathogen in swine, a PCR method was established by using a set of primers derived from the published nucleotide sequence of the PCV-2 genes deposited in GenBank. In order to confirm its specificity, the PCR was performed by using the template DNA from the reference virus, and the expected products of 536 bp were only detected in PCV-2, whereas no specific band was detected in other virus such as PRV, CSFV, PPV and PK-15 cells. The lowest DNA consistence which could be detected by the PCR method was 29 ng/L, and the sequence of aim band was belong to PCV-2. To further investigate the prevalence of PCV-2, 91 clinic samples were obtained from Shanghai and circumjacent regions in 2008, and submitted to PCR detection. The data indicated that PCV-2 in 52 samples were positive, while positive proportion was 57.14%. The clinical experiments indicate the PCR method could be used in diagnosis and epidemiology investigating.

Key words: PCV-2; PCR; detection

CLC Number: