山羊KIFI基因分子克隆及序列分析

›› 2010, Vol. 37 ›› Issue (4): 91-93.

• 生物技术 • Previous Articles Next Articles

Cloning and Sequence Analysis of Goat KIFI Gene

LI Li-juan¹，SONG De-rong²，HAN Fen-xia³

（1.Pratacultural Ecological Institute of Bijie University， Bijie 551700， China;2.Science Institute of Bijie Region Animal Husbandry and Veterinary in Guizhou Province，Bijie 551700， China;3.College of Animal Science and Technology，Henan Institute and Technology，Xinxiang 453003， China）

Received:1900-01-01 Revised:1900-01-01 Online:2010-04-20 Published:2010-04-20
Contact: SONG De-rong

Abstract

Abstract: A pair of pecific primer was designed using Primer 3.0 software to amplify the sequence of KIFI gene from goat genomic DNA. The KIFI was cloned into pGEM-T vector，positive colonies were sequenced and digested by restriction endonuclease after transforming. The results showed that the sequence length was 472 bp (No.GQ988385)，including Exon 1，part of 5′UTR and part of Intron 1 sequence of goat KIFI Gene，Exon 1 sequence coding for polypeptide contains 116 amino acid. Which shared 98%，90%，90%，88% and 84% homology with the sequences of sheep，cattle，horse，human and house mouse. Therefore，cloning of goat KIFI gene could be a basis for the further study on polymorphism and correlation on cashmere trait.

Key words: goat; KIFI gene; clone; sequence analysis

CLC Number:

Q785

LI Li-juan;SONG De-rong;HAN Fen-xia. Cloning and Sequence Analysis of Goat KIFI Gene[J]. , 2010, 37(4): 91-93.

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Cloning and Sequence Analysis of Goat KIFI Gene

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