番鸭细小病毒NS2原核表达载体的构建

›› 2010, Vol. 37 ›› Issue (4): 88-90.

• 生物技术 • Previous Articles Next Articles

Construction of Protokaryotic Expressing Vector Containing NS2 of Muscovy Duck Parvovirus

RUAN Er-lei¹，YANG Li-yun³， CHEN Fang-yan²，CHEN Rui-ai¹，WANG Lin-chuan^1,3

（1.Guangdong Dahuanong Animal Health Products Co. Ltd, Xinxing 527400, China;2.College of Animal Science, South China Agricultural University, Guangzhou 510642, China;3.College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China）

Received:1900-01-01 Revised:1900-01-01 Online:2010-04-20 Published:2010-04-20
Contact: WANG Lin-chuan

Abstract

Abstract: In this study, a pair of primers were designed according to the published DNA sequence of MDPV and was used to amplify the NS2 gene of MDPV by polymerase chain reaction（PCR）. The PCR product was then cloned into the vector pMD18-T. In order to construct a prokaryotic expression vector containing the NS2 gene of MDPV, double digestion（BamH I and Sal I）were performed to deal with pMD18-T and pET-32a respectively for the directional ligation of the two fragments. The following restriction endonuclease analysis has proved the correctness of the expression vector. Construction of the prokaryotic expression vector has laid the foundation of the expression of NS2 protein and the development genetically engineering vaccine.

Key words: MDPV; NS2 gene; prokaryotic expression; vector construction

CLC Number:

RUAN Er-lei;YANG Li-yun;CHEN Fang-yan;CHEN Rui-ai;WANG Lin-chuan;. Construction of Protokaryotic Expressing Vector Containing NS2 of Muscovy Duck Parvovirus[J]. , 2010, 37(4): 88-90.

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Construction of Protokaryotic Expressing Vector Containing NS2 of Muscovy Duck Parvovirus

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