›› 2010, Vol. 37 ›› Issue (4): 80-84.

• 生物技术 • Previous Articles     Next Articles

Determination of Content and Entrapment Efficiency for Ivermectin Liposomes by HPLC

SHE Yong-xin1，Badsang wangdui1，SE Zhu1， HE Shu-hai3，WANG Jing2，DONG Lu-de1，Laba cidan1，YANG De-quan1   

  1. (1.The Institute of Veterinary and Animal Husbandry for Tibet Academy of Agricultural and Animal Husbandry, Lhasa 850009, China; 2.Institute of Quality Standards and Testing Technology for Agri-products, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 3.Xinyang Agricultural College of Henan Province, Xinyang 464000, China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-04-20 Published:2010-04-20

Abstract: To establish a method for the determination of the content of Ivermectin and the entrapment efficiency of Ivermectin in the liposomes. Waters surfun C18 column (150 mm×2.1 mm, 5 μm) was employed, and the mobile phase consisted of acetonitrile∶methanol∶water (62∶30∶8). The detective wavelength was set at 245 nm. The flow rate was 1.0 mL/min, and the column temperature was at 30 ℃. Four kinds of methods were used for the separation of Ivermectin liposomes and free Ivermectin. There was good resolution between Ivermectin and the excipients by adopting to ultracentrifugation under this chromatographic condition. The calibration curve was good linear relation the range of 1 to 50 μg/mL. The mean recovery was 98.54%. The method was sample, accurate, sensitive and applicable for the determination of content and entrapment efficiency of Ivermctin liposomes.

Key words: Ivermectin; liposomes; entrapment efficiency; HPLC; ultracentrifugation method

CLC Number: