Abstract: PCR technique has features of rapid, sensitive and friendly operations in the detection of microorganisms. Multiplex PCR method which can simultaneously amplificate multiple target genes is a very useful method for efficient and rapid detection of food microbes and has become a popular area in food research recently. In this paper ,the multiplex PCR applied in the detection of food-born pathogenic microorganism, non-pathogenic microorganism, and food related environmental microorganism were reviewed and its application in the future research for food microbe detections were proposed.

Key words: multiplex PCR; food; microorganisms detection