›› 2010, Vol. 37 ›› Issue (10): 76-80.

• 生物技术 • Previous Articles     Next Articles

Isolation of Swine Corynebacterium and its Identificationby Analysis of 16S rRNA Gene

LI Min1, TIAN Ming-xing1, ZOU Nian-li1, WANG Yuan-ping1, LIU Ping1, HUANG Yong1,2   

  1. （1.College of Veterinary Medicine,Sichuan Agricultural University, Ya’an 625014, China;2.Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Ya’an 625014, China）
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-10-20 Published:2010-10-20
  • Contact: HUANG Yong

Abstract: An infectious disease mainly characterized of fervescence, cyanosis skin, lymphadenectasis, swollen lungs, splenomegaly, bleeding and purulent occurred in a breeding farm in Ya’an in the province of Sichuan. A pathogen was isolated by blood agar culturing, and characterized by morphological observation, biochemical test, antibiotic sensitive test and 16S rRNA gene sequence analysis. The 16S rRNA gene sequence was compared with sequences of other 25 reference bacteria belonging to Corynebacterium. The results showed that the isolated bacteria was G+, rod-shaped,and sensitive to the cephalosporin antibiotics,the homology analysis showed that the homology between isolated bacterium and other strains of Corynebacterium was from 78.2% to 98.0%. Through phylogenetic analysis, the isolated bacteria was identified as Corynebacterium.

Key words: Corynebacterium; isolation; identification; 16S rRNA; homology analysis

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