Abstract:

Estrogen receptor (ESR), follicular-stimulating hormone beta subunit ( FSHβ), and osteopontin (OPN) gene were regarded as candidate genes for reproductive traits of Large White pig and Landrace pig in this study, and the polymorphisms of these genes were detected by electrophoresis method and PCR-RFLP with restriction endonuclease Pvu Ⅱ. The results showed that: ① In Landrace pig population, allele A was the dominant gene of ESR and OPN, and the allele B was the dominant gene of FSHβ. Among the first parity sow group, the TNB, NBA,WB of BB genotype individuals of ESR gene were larger than AA genotype individuals, TNB, NBA,TWB of AA genotype individuals of FSHβ gene were larger than BB genotype, and TNB of BB genotype individual of OPN gene was 1.20 piglets less than AA genotype individuals（P＜0.05）. In multi-parity sow group, TNB and NBA of AA genotype individuals of OPN and FSHβ genes were larger than BB genotype individuals. ② In Large White pig population, allele A was the dominant gene of ESR, and the allele B was the dominant gene of FSHβ and OPN gene. Among the first parity sow group, the TNB and NBA of AA genotype individuals of FSHβ and OPN gene were larger than BB genotype individuals, the NBA and BW of ESR AA genotype were more than BB genotype. In multi-parity sow group, TNB and NBA of BB genotype individuals of OPN,ESR and FSHβ genes were larger than AA genotype individuals.

Key words: pig; PCR-RFLP; reproductive traits; estrogen receptor gene; follicular-stimulating hormone beta subunit gene; osteopontin gene