›› 2010, Vol. 37 ›› Issue (10): 130-134.

• 遗传繁育 • Previous Articles     Next Articles

Screening of Differentially Expressed Genes in Pig Parthenogenetic Embryos with Different Developmental Competence

LU Fang1，2，LUO Xue-ming2，LONG Chuan2，WANG Xi-biao1，PAN Deng-ke2   

  1. (1.College of Animal Science and Technology, Northeast Agricultural University，Harbin 150030，China;2.The Key Laboratory of Farm Animal Genetic Resources and Utilization of Ministry of Agriculture，Institute of Animal Sciences，Chinese Academy of Agricultural Sciences，Beijing 100193，China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-10-20 Published:2010-10-20
  • Contact: PAN Deng-ke

Abstract: The study obtained developmental competent and incompetent two-cell stage pig parthenogenetic embryos based on different first cleavage time and the level of maternal mRNAs was compared. Differential display reverse transcription polymerase chain reaction was carried out on maternal mRNAs isolated from these embryos. Eight fragments (E1 to E4, L1 to L4) differentially expressed between the two groups by reverse Northern blotting. After cloning and sequencing, eight homologue sequences were retrieved from NCBI database: six ESTs, E3 had high homology (the homology was 97%) with human CPEB2 and L3 had high homology (the homology was 89%) with hnRNPA1. CPEB2 and hnRNPA1 might play major roles in the developmental competence of embryos.

Key words: pig; parthenogenetic embryo; first cleavage time; maternal mRNA; embryo quality; DDRT-PCR

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