›› 2009, Vol. 36 ›› Issue (9): 135-138.

• 疾病防治 • Previous Articles     Next Articles

Development and Application of PCR Assay for Porcine Pleuropneumonia

WANG Hai-li1,2, GE Chang-cheng1, XU Gong-yi1,2, LV Yun-peng1   

  1. (1.Liaocheng Vocational and Technical College, Liaocheng 252000, China;2.China Agricultural University, Beijing 100193, China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2009-09-20 Published:2009-09-20
  • Contact: XU Gong-yi

Abstract: To study of porcine pleuropneumonia epidemic status in Liaocheng, a PCR method of detection Actinobacillus pleuropneaumoniae(App)was established. Experimental study on 186 lesion tissue samples of death pigs and 545 nasal swab samples of normal pigs, and the App positive rate were 43.0%(80/186) and 9.4%(51/545) respectively.The PCR method of detection App becomes the main way of clinical diagnosis. The survey indicates the epidemic status and inapparent infection of App were exist in Liaocheng.

Key words: Actinobacillus pleuropneaumoniae; PCR; detection

CLC Number: