Abstract: Five Newcastle disease virus strains were isolated from chickens and pigeon of poultry farm in Xinjiang. The results of the hemagglutination test (HA) and hemagglutination inhabition test （HI） indicated that the 5 NDV strains had HA. The HA of strains all can be inhibited by NDV positive serum and can’t be inhibited by AIV (H5 subtype and H9 subtypes) positive serum. In this study, the F gene of strain XJ/3/07, strain XJ/7/07, strain XJ/9/08, strain XJ/10/08 and strain XJ/11/08 were amplified by RT-PCR and cloned. The sequence analysis showed that the full-length gene of F were 1662 bp, 1589 bp, 1676 bp, 1589 bp and 1662 bp and encoding 553,528,553,520 and 553 amino acids respectively. According to the analysis 〖JP+3〗of amino acid sequence of the cleavage site of F protein, the NDV strain XJ/10/08 was virulent and the others NDV strains were avirulent. Comparison the five NDV strains with the vaccine strain La Sota and strain TaiWan95, the nucleotide homology were 83.7% to 99.8% and 84.7% to 93.3%. The phylogenetic analysis suggested that the strain XJ/3/07, strain XJ/7/07, strain XJ/9/08 and strain XJ/11/08 were gene type Ⅱ, and the strain XJ/10/08 strain was gene type Ⅶ.

Key words: Newcastle disease virus; F gene; sequence analysis