鸭新城疫病毒山东株的分离鉴定

Abstract

Abstract: The virus isolated from duck was identified as Newcastle disease virus by the HA/HI. The intracerebral pathogenicity index in 1-day-old chickens, the intravenous pathogenicity 6-week-old chickens were 1.79 and 2.45 according to the standard and methods of Newcastle disease pathogenicity judgment refer to OIE. We amplified its fusion protein gene by RT-PCR using the primer which got from the reported sequence of NDV in GenBank, cloned into the carrier and sequenced. The results showed that the isolated virus was the strong virulence NDV.

Key words: NDV from duck; isolation and identification; F gene; RT-PCR; sequence analysis

CLC Number:

S854

ZHANG Tai-xiang;WANG Bing-jun;WANG Yu;TIAN Guo-hua;TIAN Guo-ning;ZHANG Jin-ling;HAN Guang-lai;HAN Liang;ZHANG Li-ping. Isolation and Identification of Newcastle Disease Virus in Duck in Shandong Province[J]. , 2009, 36(7): 148-151.

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Isolation and Identification of Newcastle Disease Virus in Duck in Shandong Province

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