牛精子蛋白胶内酶切条件优化及问题分析

›› 2009, Vol. 36 ›› Issue (7): 106-111.

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Optimization and Analysis of In-gel Digestion with Bovine Spermatozoa Protein

HAN Wei-dong^1,2， WANG Dong²， ZHU Hua-bin²， HAO Hai-sheng²， DU Wei-hua²， ZHAO Xue-ming², WANG Zong-li¹

(1.Faculty of Animal Science & Technology, Gansu Agricultural University, Lanzhou 730070, China；2.Institute of Animal Science, Chinese Academy of Agricultural Sciences， Beijing 100193, China)

Received:1900-01-01 Revised:1900-01-01 Online:2009-07-20 Published:2009-07-20
Contact: WANG Zong-li

Abstract

Abstract: The achievemet ratio of identifying protein of sliver stained through MALDI-TOF-MS is low. The following variables were studied to effect of in-gel digestion of silver-stained 2-D gels with bovine spermatozoa protein :enzyme concentration, degestion time and dry gel. And optimization of in-gel digestion of silver-stained 2-D gels is including spot excision, destaing, digestion and extraction. Contaminating protein was analyzed and solution in this paper in order to enhance achievement ratio of protein identity

Key words: two-dimenional gel electrophoresis; in-gel digestion; proteomics

CLC Number:

HAN Wei-dong;WANG Dong;ZHU Hua-bin;HAO Hai-sheng;DU Wei-hua;ZHAO Xue-ming;WANG Zong-li. Optimization and Analysis of In-gel Digestion with Bovine Spermatozoa Protein[J]. , 2009, 36(7): 106-111.

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Optimization and Analysis of In-gel Digestion with Bovine Spermatozoa Protein

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