口蹄疫病毒灭活疫苗中VP1基因RNA抽提方法的建立

›› 2009, Vol. 36 ›› Issue (2): 58-61.

• 生物技术 • Previous Articles Next Articles

The Isolation Method Establishment of VP1 Gene RNA in Inactivated  Vaccine against FootandMouth Disease

ZHAO Yi1,2,LEI Zhen1,2，WANG Yuhong3,LIU Hong3,MA Wenge1,HUANG Jiong1,3，FU Zihua1,YI Zhong1,XUE Ying1,3

(1.Veterinary Research Institute,Xinjiang Academy of Animal Sciences, Urumqi 830000, China;2.Xinjiang Agriculture University, Urumqi 830000, China; 3.Xinjiang Tecon Animal Husbandry Biotechnology Co., Urumqi 830000, China)

Received:1900-01-01 Revised:1900-01-01 Online:2009-02-20 Published:2009-02-20
Contact: HUANG Jiong

Abstract

Abstract:

Inactivated vaccines against foot andmouth disease gathered from different manufacturers were screened by various methods.Gene VP1 RNA in live virus, inactivated vaccine and inactivated virus were isolated, RTPCR amplified, cloned, cDNA sequenced and comparative analysis. The results indicate that,VP1 RNAs isolated from live virus, virus inactivated by BEI and inactivated vaccine emulsified by white oil access to all of the 813 bp specific objectives of fragments, and the expected 813 bp fragment match. Nucleotide sequence revealed that  VP1 homology of the virus was 100% isolated by three methods. BEI and the emulsification process do not damage whole RNA structure of VP1. The isolated method to RNA can be used to analyse sequence of VP1 RNA, which derived from either live or inactivated Footandmouth Disease virus.

Key words: footandmouth virus; inactivated vaccine; BEI;  VP1 gene

CLC Number:

S852.65+9.6

ZHAO Yi;LEI Zhen;WANG Yuhong;LIU Hong;MA Wenge;HUANG Jiong;FU Zihua;YI Zhong;XUE Ying;. The Isolation Method Establishment of VP1 Gene RNA in Inactivated  Vaccine against FootandMouth Disease[J]. , 2009, 36(2): 58-61.

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The Isolation Method Establishment of VP1 Gene RNA in Inactivated  Vaccine against FootandMouth Disease

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