猪源A型多杀性巴氏杆菌灭活疫苗制备及免疫效果评估

doi:10.16431/j.cnki.1671-7236.2023.09.031

Abstract

Abstract: 【Objective】 The aim of the experiment was to prepare porcine Pasteurella multocida serotype A inactivated vaccine to effectively control the occurrence and prevalence of pasteurellosis.【Method】 Pasteurella multocida serotype A was isolated from diseased swine samples,and the growth curves were determined.Then the vaccine candidate strains were screened by pathogenicity test in mice.The obtained vaccine candidate strains were cultured and inactivated.After the sterility test was qualified,the inactivated vaccine were prepared by mixing with SUMMIT-CB-200 adjuvant in a ratio of 1∶4.The vaccine protection ability was evaluated by immune challenge protection tests in mice and piglets respectively.【Result】 11 strains of porcine Pasteurella multocida serotype A were isolated.The growth curve results showed that 11 strains reached a stable stage when cultured for 6 h,and the number of viable bacteria ranged from 2.1×10⁹ to 8.4×10⁹ CFU/mL.The pathogenicity test of mice showed that after challenge,the mice showed depression,decreased appetite,slow action,rough coat,clumping,large purulent secretions around the eyes,abdominal breathing,ataxia and other symptoms.Four vaccine candidate strains A4,A8,A9 and A11 were screened.The strongest strain A4 had a subcutaneous challenge median lethal dose (LD₅₀) of 167 CFU in mice.The results of virulence test of piglets showed that depression,loss of appetite,skin redness,vomiting,dyspnea,abdominal breathing,elevated body temperature,joint enlargement,lameness and other symptoms after challenge.The strain A4 had a subcutaneous challenge LD₅₀ of 1.72×10¹⁰ CFU.The results of immune challenge protection test showed that the four strains of inactivated vaccine had certain protective effect on mice and piglets,among which A9 had the strongest protection,with a protection rate of 60% for mice and 75% for piglets.【Conclusion】 The inactivated vaccine prepared in this study could provide better immune protection against Pasteurella multocida serotype A,which had certain development value.

Key words: porcine Pasteurella multocida; serotype A; inactivated vaccine; protecting force

CLC Number:

S852.61^＋2

HE Ya, WANG Zhaohe, CHEN Bo, SONG Yuping, ZHOU Mingguang, XU Gaoyuan. Preparation of Inactivated Vaccine Against Porcine Pasteurella multocida Serotype A and Evaluation of Its Immune Effect[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(9): 3752-3761.

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Preparation of Inactivated Vaccine Against Porcine Pasteurella multocida Serotype A and Evaluation of Its Immune Effect

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