林下菊苣草地放养对北京油鸡盲肠菌群结构和代谢物的影响

doi:10.16431/j.cnki.1671-7236.2025.01.012

Abstract

Abstract: 【Objective】 This experiment was conducted to analyze the effects of grazing on chicory grassland under forest and traditional cage system on caecum microflora and metabolites of Beijing-You chickens. 【Method】 10-week-old Beijing-You chickens with similar weight were randomly divided into three groups and raised for 95 days:Grazing on chicory grassland under forest groups,with grazing densities of 100 hens/677 m² (T1) and 120 hens/677 m² (T2),and the traditional cage system was used as the control group (CK),the grazing groups adopted a combination of grassland grazing and basal diet supplementation,and the CK group adopted basal diet only.At the end of the experiment,caecum contents were collected,the richness,diversity and structure of microbial community were analyzed by 16S rRNA high-throughput sequencing,and the differential metabolites and KEGG enrichment pathways were analyzed by liquid chromatography-mass spectrometry (LC-MS). 【Result】 Compared with CK group,the Sobs index,Chao1 index,Ace index and Shannon index of T1 group were increased,and the Simpson index was decreased to a certain extent (P＞0.05),the Sobs index,Chao1 index and Ace index of T2 group were obviously increased by 41.3%,33.6% and 34.7% (P＜0.05).Compared with CK group,the relative abundance of Ruminococcus_torques_group,Rikenellaceae_RC9_gut_group,Olsenella,unclassified_f__Lachnospiraceae and UCG-005 in caecum of T1 and T2 groups were increased to a certain extent (P＞0.05).The difference metabolites of T1 and T2 groups were mainly concentrated in the metabolism of terpenoids and polyketides,biosynthesis of plant secondary metabolites,lipid metabolism and amino acid metabolism. 【Conclusion】 The relative abundance of beneficial bacteria in caecum and the lipid metabolism pathway and amino acid metabolism pathway of Beijing-You chickens were positively affected by grazing on chicory grassland under forest,and it played a series of benign effects such as controlling body fat,increasing essential amino acid and improving immune performance.

Key words: gazing on grassland under forest; Beijing-You chickens; caecum microflora; caecum metabolites

CLC Number:

S831.4

ZHANG Suhan, MAO Peichun, TIAN Xiaoxia, GUO Yuxia, ZHENG Mingli, MENG Lin. Effects of Grazing on Chicory Grassland Under Forest on Caecum Microflora and Metabolites of Beijing-You Chickens[J]. China Animal Husbandry and Veterinary Medicine, 2025, 52(1): 133-146.

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Effects of Grazing on Chicory Grassland Under Forest on Caecum Microflora and Metabolites of Beijing-You Chickens

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[1]	ZHANG Suhan, MENG Lin, GUO Yuxia, ZHENG Mingli, MAO Peichun, TIAN Xiaoxia. The Effects of Grazing on Chicory Grassland Under Forest on the Slaughter Performance, Egg Quality, Meat Quality, Lipid Metabolism and Immune Performance of Beijing-You Chickens [J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(1): 114-124.
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