猪链球菌2型LUX荧光PCR检测方法的建立

Abstract

Abstract: The infection caused by Streptococcus suis type 2 is an important zoonosis. Based on the conserved cps2J region of Streptococcus suis type 2, the LUX primers were designed and the LUX PCR assay was developed to detect Streptococcus suis type 2. The sensitivity and specificity of the developed assay were analyzed by using the tonsils samples artificially contaminated with plasmid DNA as testing materials. Results showed that the developed Streptococcus suis serotype 2 specific LUX PCR assay was sensitive enough to detect 100 copies of plasmid DNA from the mask samples. The assay could distinguish Streptococcus suis serotype 2 from Streptococcus agalactiae, swine Pasteurellosis mutocida, Streptococcus faecalis, Streptococcus suis equi subsp equi, swine Cholerasuis and other swine bacteria pathogens. The developed assay can serve a candidate for the quick detection of Streptococcus suis serotype 2 infection in the field.

Key words: Streptococcus suis serotype 2; LUX; PCR; detection

CLC Number:

S852.61

WANG Cai-xia;ZHA Cheng-gang;WU Shao-qiang;LIN Xiang-mei. Development of a LUX PCR Assay for Detection of Streptococcus suis Type 2[J]. , 2009, 36(12): 45-48.

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Development of a LUX PCR Assay for Detection of Streptococcus suis Type 2

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