Abstract: To determine the responses of dairy cow performance by diets contained diferent level cottonseed, thirty two primiparous Holstein cows was divided into four groups according to random split plot design by milk yeild, body weight and milking period. Animals in control, group 1, group 2 and group 3 was fed with diets contained 0%, 8%, 12% and 16% conttonseed, respectively. The milk yeild of group 1 was 5.65% more than control(P<0.05). The milk fat yeild of group 1 was significant higher than the others(P<0.05). There was difference of dry matter intake among groups.

Key words: cottonseeds; matter intake; milk yeild; milk fat; milk protein