牛奶和山羊奶中乳脂球膜蛋白的比较研究

doi:10.16431/j.cnki.1671-7236.2016.11.023

Abstract

Abstract:

In order to compare the difference of milk fat globule membrane（MFGM）proteins between dairy cow and goat milk,30 milk samples were collected in a dairy farm and a goat farm from Anhui area,respectively.Extracted proteins from the MFGM-enriched fractions were identified and quantified by LC/MS approach.The results showed that 284 and 334 proteins of MFGM from dairy cow and goat were identified,the biological processes of MFGM proteins were similar between dairy cow and goat which were mainly related to biological regulation,localization,transport,signal transduction and response to stimulus.Meanwhile,there were some differences in molecular functions,and protein binding and nucleotide binding were the most prevalent molecular functions in dairy cow MFGM proteins,while protein binding and structural molecule activity were the most prevalent molecular functions in goat MFGM proteins.And structural molecule activity was the main molecular functions among the difference proteins.Pathway analysis revealed that tight junction,axon guidance,antigen processing and presentation,complement and coagulation cascades were enrichment in dairy cow MFGM proteins,and regulation of actin cytoskeleton was enrichment in goat MFGM proteins.Those results revealed the protein expression pattern difference between MFGM protein of dairy cow and goat milk,and provide reference data for further exploring the molecular mechanism of synthetic milk fat globule.

Key words: milk fat globule membrane; LC/MS; dairy cow milk; goat milk

CLC Number:

TS252.2

ZHAO Xiao-wei, YANG Yong-xin, HUANG Dong-wei, CHENG Guang-long, ZHAO Hui-ling. Comparison of Milk Fat Globule Membrane Proteins in Milk Samples of Dairy Cow and Goat[J]. , 2016, 43(11): 2963-2969.

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Comparison of Milk Fat Globule Membrane Proteins in Milk Samples of Dairy Cow and Goat

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