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Table of Content

20 June 2008, Volume 35 Issue 6
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动物营养与饲料科学
The Effect of Diets Contained Different Cottonseed on Peformance of Lactation Dairy Cows
ZHU Wen-tao;WANG Jia-qi;WANG Jian-ping;YANG Kai-lun;BU Deng-pan
2008, 35(6):  5-8. 
Abstract ( 1800 )  
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To determine the responses of dairy cow performance by diets contained diferent level cottonseed, thirty two primiparous Holstein cows was divided into four groups according to random split plot design by milk yeild, body weight and milking period. Animals in control, group 1, group 2 and group 3 was fed with diets contained 0%, 8%, 12% and 16% conttonseed, respectively. The milk yeild of group 1 was 5.65% more than control(P<0.05). The milk fat yeild of group 1 was significant higher than the others(P<0.05). There was difference of dry matter intake among groups.
The Studies on the Energy and Protein Requirements of Fattening Crossbred Boer Goats
LIANG Xian-wei;YANG Bing-zhuang;BAO Fu-yin;ZOU Cai-xia;LIANG Kun;ZHENG Wei;ZHENG Hong-ying;LI Li-li;ZOU Long-shu
2008, 35(6):  13-17. 
Abstract ( 799 )  
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Fattening period through research crossbred Boer goats of the wave energy and protein nutritional requirements parameters, screening of a better economic efficiency and the energy mix of protein feed supplements, with a view to keeping the scientific theoretical basis and provide production guidance. Sixteen fattening crossbred male Boer goats F1 weaned at the age of 3 month were used to study . A 4×4 Latin square experiment design was conducted to study the energy and protein requirements of stallfeeding crossbred Boer goats fed the four different diets based on recommendation of nutrients requirements of NRC(1981).The results indicated that four different diets ration the relationship between DMI and average daily gain (ΔW,g/d) and metabolic weight (W0.75,kg) is:DMI (g/d)=181.3W0.75－.61ΔW－886.2（r=0.9287）.The prediction equations of nutritional requirements about gross energy (GE), digestible energy (DE) and crude protein (CP) for stallfeeding growing crossbred Boer goats were:CP（g/d）=19.56 W0.75+0.25ΔW-128.6（r=0.7836），GE（MJ/d）=2.98 W0.75+0.023ΔW-18.69（r=0.8257），DE（MJ/d）=1.26 W0.75-0.006ΔW-3.56（r=0.6236）。For the stallfeeding growing crossbred Boer goats, aged at 3 to 6 months, Metabolic rate of gross energy of the diets is 0.49; digestibility of crude protein is 0.63.The protein requirement of per live weight gain form eat goats is 0.32g PD/g gain (PD, protein deposited).
Effect of Concentration Echinacea on Antioxidant Function of Broilers
GAO Yan-ni;YUAN Ying;WANG Ji-lei;ZHAI Feng
2008, 35(6):  18-20. 
Abstract ( 1568 )  
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One hundred and eighty avin broilers were divided into 6 groups with oneway random design, each group including 3 replicates (10 briolers per replicate) .The first group was the control group, fed with cornsoybean and the Second to 6th groups were experimental groups, fed with basal diet supplement with 5，10，50，100，500 mg/kg Echinacea respectively. The whole experimental period was 7 weeks. The objective was to study the effects of different concentration Echinacea on antioxidant function and production performance. The experimental results showed that: The Echinacea abstract with 50mg/kg had the highest free radical scavenging effects on hydroxyl free radicals，and the activities of SOD，GSHPx were enhanced，the content of MDA in blood serum of the broilers was decreased. But there is not significantly different in the production performance ,just a certain improvement.
The Functions of the Trance Element Zinc in Animal Product
FENG Bing-fu;;ZHAO Xin-quan-;CAO Jun-hu;
2008, 35(6):  26-28. 
Abstract ( 1498 )  
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As a necessary trace element in animal nutrition, the functions of zinc on animal production have been universally and extensively investigated. In this paper, the metabolism in animal, the functions on animal production and the problems in study of it were summarized. 
生物技术
Clonging and Sequence Analysis of the Interferon-α Gene of Shitou Goose
ZHENG Xiao-ling;LU Xi;LIU Yan-fen;CHEN Shao-hong;YANG Le-le;LIU You
2008, 35(6):  29-33. 
Abstract ( 1549 )  
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With nested transcription polymerrase chain reaction, the interferon-α gene fragment of shitou goose (d-Go-IFN-α) was amplified from genome DNA extracted from peripheral blood cells (PBCs). At the same time, the total RNA was extracted from peripheral blood lymphocyte (PBLs) stimulated with Newcasttle disease virus(NDV) in vivo, and transcription polymerrase chain reaction(RT-PCR) was used to amplify the interferon-α gene fragment of shitou goose (m-Go-IFN-α), then both of the amplified fragments were cloned into pGEM-T easy vector and sequenced. The results showed that both of the d-Go-IFN-α and m-Go-IFN-α were both consisted of 576 necleotides, coding for 192 amino acids. Compared with the nucleotide sequences of d-Go-IFN-α and m-Go-IFN-α, a variation A→G at the 155th site was observed, and the 52th amino acid was mutated from M to R accordingly, which implied that there was single nucleotde polymorphism in IFN-α sequence.of Shitou goose. The homolony of nucleotide and the deduced amino acid sequence among different poultry breeds were 41.2 % to 98.3% and 13.0% to 96.4% respectively. The secondary structure of the IFN-α were almost the same among different goose breeds. 
The Establishment of AFLP Technique System for Superfine Wool Sheep
HE San-gang;HUANG Xi-xia;TIAN Ke-chuan;SHI Hong-caiWEI Xia;XU Xin-ming;ZHANG Yan-hua
2008, 35(6):  40-42. 
Abstract ( 1300 )  
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An AFLP(amplified fragment length polymorphism) analysis system was established with Xinji superfine Merino sheep by optimizing of AFLP technical system ,including enzyme digestion, adapter ligation ,preamplification ,selective amplification, denatured polyacrylamide gel electrophoresisa and silver staining .
遗传繁育
The Polymorphism of DRD1 Gene and Its Relationship with Selfbiting Behaviors in American Mink
LIN Ning;NING Fang-yong;WANG Guang-sheng;BAI Xiu-juan
2008, 35(6):  52-55. 
Abstract ( 1616 )  
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We report the effects of candidate gene Dopamine Receptor D1 (DRD1) gene on selfbiting in the current study. In this study, partial sequence of Dopamine Receptor D1 genes were firstly extracted from feet in American mink, and were amplified using Polymerase Chain Reaction (PCR) method, then sequenced. Now the sequence has been sent to Gene Bank with registration, and the Accession Number is EU249297. Comparison the fragment sequence of different individuals, we found T→C mutation, but coded amino acid had no change. Then followed by single strand conformation polymorphism (PCR-SSCP) analysis, there showed three kinds of genotypes: AA, AB, BB. The correlation between the genotypes with the in selfbiting behavior was analyzed, the results showed that the frequency of allele A is higher than allele B, the AA genotypes in control group were higher than that of selfbiting group, and is different significantly (P<0.05).
Introduction on Major Genes of Some High Prolific Sheep Abroad
CHENG Du-xue;CHU Ming-xing;LIU Wen-zhong
2008, 35(6):  56-58. 
Abstract ( 1382 )  
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Reproductive traits are important economic traits in sheep. This review introduced reproductive characteristics of some high prolific sheep breeds abroad and the discovery, genetic effect, chromosome mapping of relevant major genes in these sheep breeds.
Application of Mitochondrial DNA in Domestic Animals Genetics and Breeding
CUI Kui-qing
2008, 35(6):  59-63. 
Abstract ( 1347 )  
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he research of animal’s mitochondrial genome is proved to be an important model branch among molecular genetics and systemic evolution. The study of mtDNA on domestic animal and its application on genetic polymorphism research are presented in this paper.
2008, 35(6):  64-65. 
Abstract ( 1160 )  
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Research on Progress of Breeding for Disease Resistance in Pig
SU Guang-hua
2008, 35(6):  66-68. 
Abstract ( 1356 )  
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Disease loss 12％ to 15％ gross production of pig industry.Improving pig disease resistance and establishing breed for disease resistance became research focus.This article reviewed research progress of IFN gene NRAMP1 gene ETEC MHC gene which are candidate gene of disease resistance pig.
论文
Effect of Traditional Chinese Medicine on Growth Performance of Broiler 
TANG Guang-wu;WANG Tao;WU Yu-cheng;BAI Yan-jie
2008, 35(6):  84-85. 
Abstract ( 1063 )  
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The research choose 600 AA broilers which were randomly divided into two groups, each group repeat three times. experiment groups were fed traditional Chinese medicine group and control group were fed basal diet with 20 days. The result show: traditional Chinese medicine additive can improve growth performance of broilers in high temperuture. Experiment groups were more quiet than control groups especially in last week. Average daily gain in experiment groups can improve 8.1% than control groups（P<0.05),and feed/meat ratio in experiment group reduce 7.34% than control groups（P<0.05）.But average daily feed intake is appreciably more high in experiment groups than in control groups. In a word , traditional Chinese medicine additive can improve growth performance of broilers and reduce feed/meat ratio.
疾病防治
Detection of Liver Function Indexes in Yak with IBRV
LU Fu-shan;LIN Qing-lian
2008, 35(6):  94-95. 
Abstract ( 1496 )  
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34 serums of yak with IBRV（ positive group） and 18 serums of health （negetive group） were detected liver function indexes by mean of boichemistry. The results were showed that: The total protein, globin, βglobin of positive serum were lower than those of negative serum (P<0.05), but AST, ALT and AST/ALT of positive serum were higher than those of negative serum ,there was very significant difference (P<0.01), there were not statistical value between others indexes of positive serum and ones of negative serum（P>0.05），and livers of part yaks with IBRV were damagede heavily（11.77%）.