›› 2008, Vol. 35 ›› Issue (6): 40-42.

• 生物技术 • Previous Articles     Next Articles

The Establishment of AFLP Technique System for Superfine Wool Sheep

HE San-gang1，3,HUANG Xi-xia1,TIAN Ke-chuan2,SHI Hong-cai3
WEI Xia1, XU Xin-ming2，ZHANG Yan-hua2   

  1. (1. College of Animal Science of Xinjiang Agricultural University, Urumqi 830052，China；2. Xinjiang Academy of Animal Science, Urumqi 830000，China；3. Xinjiang Academy of Animal Science,The KeyLab of Livestock Reproduction & Breed Biotechnology of MOA，Urumqi 830000，China）
  • Received:1900-01-01 Revised:1900-01-01 Online:2008-06-20 Published:2008-06-20

Abstract: An AFLP(amplified fragment length polymorphism) analysis system was established with Xinji superfine Merino sheep by optimizing of AFLP technical system ,including enzyme digestion, adapter ligation ,preamplification ,selective amplification, denatured polyacrylamide gel electrophoresisa and silver staining .

Key words: superfine merino sheep; genome DNA; AFLP

CLC Number: