›› 2008, Vol. 1 ›› Issue (11): 65-69.

• 遗传繁育 • Previous Articles     Next Articles

An Research of the Growth Rule of Cells Cultured in Vitro of Livestock and Poultry

LU Taofeng1, LIU Changqing1,3,WU Hongmei1,ZHANG Yanyan1,4,LIU Shuai1,2,BAO Adong1,2,ZHANG Wenxiu1,GUAN Weijun1,MA Yuehui1   

  1. 1.Institute of Beijing Animal Science and Veterinary, CAAS, Beijing 100193,China;2.Inner Mongolia Agricultural University,Hohhot 010000, China;3.Division of Life Sciences and Technology，Ocean University of China, Qingdao 266003, China；4.Northwest A & F University,Yangling 712100,China
  • Received:1900-01-01 Revised:1900-01-01 Online:2008-11-20 Published:2008-11-20

Abstract: In order to study on the growth and differentiation rule of cells cultured in vitro of Luxi cattle,Mongolia horse,Peking duck and Langshan chicken,the vitro four cell lines were established successfully in this study with tissure attachment culture method. Analysis on the growth and differentiation rule of fibroblast cell through rendering the growth curve and photographing the cells growth process by Confocal Laser Scanning Microscopy. The experimental results indicate that: the four cell growth curves are all showed significantly sshape, the length of retention period and incubation period are obviously different between livestock and poultry cells , and the doubling time for livestock and poultry cells are also, in addition, the single cell division period of Mongolia horse and Langshan chicken cells cultured in vitro are obviously different too, the former is 97 min and the latter is 85 min.

Key words: livestock and poultry; fibroblast cell; growth rule; growth curve; division period

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