菊苣酸介导外泌体对巨噬细胞免疫抑制的影响

doi:10.16431/j.cnki.1671-7236.2023.10.036

Abstract

Abstract: 【Objective】 The macrophage immunosuppression model was established in vitro to investigate the effect and regulatory mechanism of cichoric acid (CA) on macrophage immunosuppression induced by phosphoramide mustard (PM),which would provide a theoretical basis for the development of CA as a new drug for clinical prevention and treatment of immunosuppressive diseases.【Method】 The macrophage-derived exosomes were extracted by ExoQuick immunoprecipitation kit.The morphology,particle size and concentration of exosomes as well as the expressions of surface marker proteins CD63 and HSP90 were identified by transmission electron microscopy (TEM),nanoparticle tracking analysis (NTA) and Western blotting.Meanwhile,macrophages were induced by PM with a concentration of 3 μmol/L for 24 h,the immunosuppression model was established,then low,medium and high doses of CA (25,50,100 μmol/L) were given for intervention.CCK-8 was used to measure cell viability,neutral red was used to measure phagocytosis ability,and NTA was used to measure exosome particle size and concentration,so as to study the mechanism of CA mediated exosomes to enhance immunity.【Result】 The extracted macrophage derived exosomes had intact cup shape,particle size between 30-200 nm,clear expression of marker proteins,and good biological activity.According to the results of CCK-8,neutral red,and NTA measurements,CA had no toxic effect on macrophages in the concentration range of 3.12-400 μmol/L.Compared with PM model group,low,medium and high doses of CA could extremely significantly improve the activity and phagocytosis of immunosuppressive macrophages (P＜0.01),promote the secretion of exosomes observably (P＜0.01).【Conclusion】 CA might activate macrophages by regulating the secretion of macrophage-derived exosomes,enhancing the cell viability and phagocytosis ability,revealing the potential mechanism of CA in enhancing immunity.

Key words: cichoric acid; macrophages; exosomes; phosphoramide mustard; immunosuppression

CLC Number:

S859.79

BAI Lixia, LIU Xiwang, YANG Yajun, QIN Zhe, LI Shihong, GE Wenbo, XU Xiao, FENG Chenjing, LI Jianyong. Effect of Cichoric Acid Mediated Exosomes on Macrophage Immunosuppression[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(10): 4223-4230.

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Effect of Cichoric Acid Mediated Exosomes on Macrophage Immunosuppression

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